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AssigneePhytowelt Greentechnologies GmbH
InventorsGuido Jach, Sanae Azdouffal, Katrin Schullehner, Peter Welters, Angela Goergen
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The present invention concerns a method of producing and enantiomerically pure alpha-ionone. Further, the invention concerns a nucleic acid that comprises a sequence that encodes a lycopene-epsilon-cyclase (EC), a lycopene-epsilon-cyclase (EC), plasmids, which encode components of the alpha-ionone biosynthesis and a microorganism that contains heterologous nucleotide sequences which encode the enzymes geranylgeranyl-diphosphate-synthase, isopentenyl-diphosphate-isomerase (IPI), phytoene desaturase-dehydrogenase (crtI), phytoene synthase (crtB), lycopene-epsilon-cyclase (EC) and carotenoid-cleavage-dioxygenase (CCD1). Further, the invention concerns a method of producing highly pure epsilon-carotene.
A method of producing enantiomerically pure alpha-ionone comprising culturing an Escherichia coli that produces isopentenyldiphosphate (IPP) and a corresponding isomer dimethyl-allyl-diphosphate (DMAPP) as starting materials for the production of enantiomerically pure alpha-ionone, and wherein the Escherichia coli further comprises one or more expression cassettes having a sequence according to one of SEQ ID NO. 43 or 44 that encode the following enzymes:
Dependencies are inferred from the claim text.