Methods and compositions for activation of innate immune responses through RIG-I like receptor signaling
AssigneeUniversity of Washington through its Center for Commercialization
InventorsMichael J. Gale, Jr., Gretja Schnell, Yueh-Ming Loo
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Abstract
Compositions and methods are provided that enable activation of innate immune responses through RIG-I like receptor signaling. The compositions and methods incorporate synthetic nucleic acid pathogen associated molecular patterns (PAMPs) that comprise elements initially characterized in, and derived from, the hepatitis C virus genome.
Claims26
An isolated nucleic acid molecule with a non-naturally occurring sequence, the isolated nucleic acid comprising:
- a 5′-arm region comprising a terminal triphosphate;
- a poly-uracil core comprising at least 8 contiguous uracil residues;
- and
- a 3′-arm region comprising at least 8 nucleic acid residues, wherein the 5′-most nucleic acid residue of the 3′-arm region is an adenine residue, and wherein the 3′-arm region is at least 30% uracil residues,
- wherein the 5′-arm region and the poly-uracil core do not naturally occur contiguously together in a Hepatitis C virus.
An isolated nucleic acid molecule with a non-naturally occurring sequence, the isolated nucleic acid comprising:
- a 5′-arm region comprising a terminal triphosphate;
- a poly-uracil core comprising at least 18 contiguous uracil residues;
- and
- a 3′-arm region comprising at least 8 nucleic acid residues, wherein the 5′-most nucleic acid residue of the 3′-arm region is a cytosine residue, and wherein the 3′-arm region is at least 30% uracil residues,
- and wherein the 5′-arm region and the poly-uracil core do not naturally occur contiguously together in a Hepatitis C virus.
Dependencies are inferred from the claim text.