Injectable microtissue systems, devices, and methods

An injectable microtissue comprising:

• a plurality of spheroids, each comprising a plurality of cells of a first cell type and a plurality of cells of a second cell type, the first cell type being endothelial cells that are concentrated in an interior core region of each spheroid;
• the plurality of spheroids being free of vascular interconnections therebetween and suspended in an injectable medicament,
• wherein the injectable microtissue is free of brown adipose tissue,
• wherein the injectable microtissue is suitable for therapeutic injection in a human patient,
• wherein a majority of the spheroids have diameters less than 200 μm, and wherein the second cell type is mesenchymal stem cells.

A method of treating a patient, the method comprising:

• injecting an injectable microtissue into a patient such that a new vascular network is formed therein with the endothelial cell core regions of the spheroids as nodes,
• wherein the injectable microtissue comprises:
• a plurality of spheroids, each comprising a plurality of cells of a first cell type and a plurality of cells of a second cell type, the first cell type being endothelial cells that are concentrated in an interior core region of each spheroid, wherein the second cell type is one of mesenchymal stem cells, smooth muscle cells, and dermal fibroblasts;
• the plurality of spheroids being free of vascular interconnections therebetween and suspended in an injectable medicament,
• wherein the injectable microtissue is free of brown adipose tissue,
• wherein the injectable microtissue is suitable for therapeutic injection in a human patient,
• wherein a majority of the spheroids have diameters less than 200 μm, and
• wherein the endothelial cells concentrated in an interior region define a distinct structure with discernable boundaries and consisting of said endothelial cells, the endothelial cells are vascular endothelial cells, and the distinct structure is a blood vessel precursor.

A method of treating a patient, the method comprising:

• injecting an injectable microtissue into a patient such that a new vascular network is formed therein with the endothelial cell core regions of the spheroids as nodes,
• wherein the injectable microtissue comprises:
• a plurality of spheroids, each comprising a plurality of cells of a first cell type and a plurality of cells of a second cell type, the first cell type being endothelial cells that are concentrated in an interior core region of each spheroid;
• the plurality of spheroids being free of vascular interconnections therebetween and suspended in an injectable medicament,
• wherein the injectable microtissue is free of brown adipose tissue,
• wherein the injectable microtissue is suitable for therapeutic injection in a human patient,
• wherein a majority of the spheroids have diameters less than 200 μm, and
• wherein the second cell type is mesenchymal stem cells.