System with buffer for lateral flow on a porous membrane
AssigneeLawrence Livermore National Security, LLC
InventorJane P. Bearinger
Life of this patent
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Abstract
Systems are described, based on a primary binding compound and a secondary binding compound used in combination with a support to detect a target in a sample. The systems includes at least one support structure, at least one small primary support portion containing at least one molecule covalently bound to a visual colloidal marker, a plurality of secondary support portions comprising secondary binding compounds that are covalently bound to the support portions and chemically active, at least one pH litmus indicator, at least one pH strip, a buffer for lateral flow on the porous membrane support that allows preservation and activity of binding compounds.
Claims11
A system comprising
- a porous membrane support structure having a lateral dimension in direction of a lateral flow and a second dimension perpendicular to the lateral dimension,
- at least one primary support portion containing first binding compounds covalently bound to visual colloidal markers, capable of binding respective targets by forming first binding compound-target complexes with the respective targets,
- secondary support portions spaced and staggered in the lateral dimension each comprising second binding compounds covalently bound to the porous membrane support structure, the second binding compounds configured to bind either the targets or the first binding compound-target complexes, each second binding compound of the second binding compounds specific to a different target or different first binding compound-target complex of the respective targets, the second binding compounds being different from and non-competitive with the first binding compounds for said target,
- control regions spaced and staggered in the lateral dimension separate from and not overlapping with the secondary support portions and in a one-to-one correspondence with the secondary support portions such that every secondary support portion is adjacent to a corresponding control region, the control regions comprising third binding compounds configured to bind a respective first binding compound of the first binding compounds when unbound to the respective target, each control region configured to provide a control for the different targets at the secondary support portions, wherein lateral placement of each control region has a same lateral placement as its corresponding secondary support,
- a pH litmus indicator and a pH control strip that is separate from and not overlapping the pH litmus indicator, wherein the pH litmus indicator and the pH control strip are both located on the porous membrane support structure in the lateral dimension downstream of the secondary support portions, wherein lateral placement of the pH control strip is such that a lateral downstream end of the pH control strip has the same lateral position as the lateral downstream end of the pH litmus indicator, and
- a liquid medium for lateral flow on the support structure, configured to allow preservation and activity of binding compounds.
Dependencies are inferred from the claim text.