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AssigneeRoche Diagnostics Operations, Inc.
InventorsBarbara Upmeier, Toralf Zarnt, Dieter Roessler, Johannes Polz
Estimating the term…
Disclosed is an immunoassay method for detecting an analyte such as an antigen or an antibody in an isolated sample suspected to contain the analyte by incubating the sample with a plurality of binding partners, one of which carries a detectable label, wherein a label-specific binding partner is added that does not carry a label but binds to the detectable label. The method is applicable for a large variety of analytes and has proven particularly useful for analyte antibodies of the IgG and IgM class present in samples due to infections by pathogens. Also disclosed is a reagent kit useful for the method comprising at least two analyte-specific binding partners one of which carries a detectable label and a label-specific binding partner that binds to said detectable label but itself does not carry a detectable label.
An immunoassay method for detecting an analyte in an isolated sample suspected to contain said analyte comprising:
A reagent kit for detecting an analyte by an immunoassay comprising a plurality of binding partners wherein at least one of the binding partners binds to the analyte, and one of the binding partners carries a detectable label and one of the binding partners is label-specific and binds to said detectable label but does not carry a detectable label, wherein the label-specific binding partner is a biological molecule based on a polypeptide composed of amino acids.
Dependencies are inferred from the claim text.