Enhanced metabolite-producing yeast
AssigneeAdisseo France S.A.S.
InventorsDominique Louis, Karine Jaillardon, Dominique Thomas
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Abstract
Metabolites produced by a microorganism using more particularly oxaloacetate as substrate or co-substrate upstream in the biosynthesis pathway. There is indeed a need in the art for transformed, in particular recombinant, microorganisms having at least an increased ability to produce oxaloacetate, thus allowing an increased capacity to produce oxaloacetate-derived amino acids and amino acid derivatives, the oxaloacetate-derived amino acids and amino acid derivatives being termed oxaloacetate derivatives. The solution is the use of a genetically modified yeast including many modifications as described in the present text.
Claims20
A recombinant yeast, in the genome of which:
- (A) at least one nucleic acid encoding a glucose-6-phosphate dehydrogenase is overexpressed and/or is under the control of an inducible or repressible promoter;
- (B) at least one nucleic acid encoding a 6-phosphogluconate dehydrogenase, decarboxylating 1 is overexpressed and/or is under the control of an inducible or repressible promoter;
- and
- (C) (i) at least one nucleic acid encoding a phosphoenolpyruvate carboxylase that converts phosphoenol pyruvate into oxaloacetate is overexpressed and/or is under the control of an inducible or repressible promoter;
- and/or
- (ii) at least one nucleic acid encoding a phosphoenolpyruvate carboxykinase that converts phosphoenol pyruvate PEP into oxaloacetate is overexpressed and/or is under the control of an inducible or repressible promoter.
Dependencies are inferred from the claim text.