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US 11,319,559 B2Granted
Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
AssigneeDsm IP Assets B.V.
InventorsJohannes Augustinus Kroon, Pierre Louis Woestenborghs
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Abstract
The invention relates to a process for the preparation of a sugar and/or fermentation product from lignocellulosic material.
Claims18
A process for the preparation of preparing at least one sugar from lignocellulosic material, comprising:
- a) pretreating the lignocellulosic material at a temperature of 160° C. to 200° C. at a pH of 1.0 to 2.5 for 1 to 15 minutes;
- b) enzymatically hydrolysing the pretreated lignocellulosic material having a dry matter weight of 15 to 25% (w/w) at a temperature of 50° C. to 65° C. and a pH of 4 to 6 for 40 hours to 150 hours using a whole fermentation broth of a filamentous fungus, said broth comprising at least a cellobiohydrolase, an endoglucanase, a beta-glucosidase, a xylanase, a beta-xylosidase, and a lytic polysaccharide monooxygenase;
- and
- c) optionally, recovering the at least one sugar from the enzymatically hydrolysed pretreated lignocellulosic material,
- wherein the at least one sugar is selected from the group consisting of:
- glucose;
- cellobiose;
- xylose;
- arabinose;
- galactose;
- fructose;
- mannose;
- rhamnose;
- ribose;
- galacturonic acid;
- glucuronic acid;
- and combinations thereof.
14 more elements
A process for the preparation of a fermentation product from lignocellulosic material, comprising:
- a) pretreating the lignocellulosic material at a temperature of 160° C. to 200° C. at a pH of 1.0 to 2.5 for 1 to 15 minutes;
- b) enzymatically hydrolysing the pretreated lignocellulosic material having a dry matter weight of 15 to 25% (w/w) at a temperature of 50° C. to 65° C. and a pH of 4 to 6 for 40 hours to 150 hours using a whole fermentation broth of a filamentous fungus, said broth comprising at least a cellobiohydrolase, an endoglucanase, a beta-glucosidase, a xylanase, a beta-xylosidase and a lytic polysaccharide monooxygenase, to obtain a hydrolysed lignocellulosic material;
- c) fermenting the enzymatically hydrolysed pretreated lignocellulosic material to produce a fermentation product;
- and
- d) optionally, recovering the fermentation product,
- wherein the fermentation product is selected from the group consisting of:
- arabinitol;
- butanol;
- ethanol;
- glycerol;
- methanol;
- 1,3-propanediol;
- sorbitol;
- xylitol;
- acetic acid;
- acetonic acid;
- adipic acid;
- ascorbic acid;
- acrylic acid;
- citric acid;
- 2,5-diketo-D-gluconic acid;
- formic acid;
- fumaric acid;
- glucaric acid;
- gluconic acid;
- glucuronic acid;
- glutaric acid;
- 3-hydroxypropionic acid;
- itaconic acid;
- lactic acid;
- maleic acid;
- malic acid;
- malonic acid;
- oxalic acid;
- oxaloacetic acid;
- propionic acid;
- succinic acid;
- xylonic acid;
- acetone;
- aspartic acid;
- glutamic acid;
- glycine;
- lysine;
- serine;
- tryptophan;
- threonine;
- ethylene;
- a β-lactam antibiotic;
- a cephalosporin;
- pentane;
- hexane;
- heptane;
- octane;
- nonane;
- decane;
- undecane;
- dodecane;
- cyclopentane;
- cyclohexane;
- cycloheptane;
- cyclooctane;
- pentene;
- hexene;
- heptene;
- octane;
- methane;
- hydrogen (H2);
- carbon dioxide (CO2);
- carbon monoxide (CO);
- a protease;
- a cellulase;
- an amylase;
- a glucanase;
- a lactase;
- a lipase;
- a lyase;
- an oxidoreductase;
- a transferase;
- and a xylanase.
76 more elements
Dependencies are inferred from the claim text.