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US 11,319,530 B2Granted
Host cells and systems for membrane protein expression
AssigneePresident and Fellows of Harvard College
InventorsJonathan Beckwith, Dana Boyd, Jessica Blazyk, Feras Hatahet
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Abstract
Provided herein are host cells or host cellular expression systems that express a membrane protein. Also, methods are provided that use such host cells or host cellular expression systems to produce higher amounts of the membrane proteins. Further, the cells or cellular systems can be used as tools for the functional characterization of membrane proteins, as well as for screening and drug discovery efforts targeting membrane proteins.
Claims11
A host cell comprising:
- an exogenous nucleic acid molecule encoding a membrane protein, said membrane protein comprises at least one mutation;
- and
- wherein the host cell comprises a double mutation at T362I and T373I, or a double mutation at T362I and G512S, in the gene yidC.
A host cell comprising:
- an exogenous nucleic acid molecule encoding a membrane protein, wherein said membrane protein comprises at least one mutation, wherein the membrane protein is vitamin K epoxide reductase (VKORc1);
- and
- wherein the host cell comprises a double mutation at T362I and T373I, or a double mutation at T362I and G512S, in the gene yidC.
Dependencies are inferred from the claim text.