Carotenoid and amino acid biosynthesis using recombinant corynebacterium glutamicum
AssigneeUniversitäT Bielefeld
InventorsPetra Peters-Wendisch, Volker Wendisch, Nadja Alina Henke
Life of this patent
Estimating the term…
Abstract
The present invention provides a method of producing astaxanthin and lysine in recombinant gram-positive bacteria comprising a nucleic acid sequence encoding for a crtZ-protein from F. pelagi and comprises a nucleic acid sequence encoding for a crtW-protein.
Claims13
A process for the preparation of astaxanthin and lysine in recombinant C. glutamicum, wherein the genome of said recombinant C. glutamicum comprises a deletion in the endogenous crtR, crtY and crtEb genes, and wherein said recombinant C. glutamicum has been genetically modified to introduce crtE, crtB, and crtl genes from C. glutamicum, a crtY from P. ananatis, at least one recombinant gene encoding a crtZ-protein from F. pelagi, B. bacteroides, S. astaxanthinifaciens, or B. vesicularis, and at least one recombinant gene encoding a crtW-protein from F. pelagi, B. aurantiaca, S. astaxanthinifaciens, B. bacteroides, or B. vesicularis.
Dependencies are inferred from the claim text.