US 11,312,980 B2 · Method for producing 3-hydroxy-4-aminobenzoic acid compound

Abstract

Provided is a method for manufacturing a 3-hydroxy-4-aminobenzoic acid by using a microorganism. The method for manufacturing a 3-hydroxy-4-aminobenzoic acid comprises a step of bringing a 4-aminobenzoic acid into contact with a microorganism that produces the following polypeptide (A) or (B): (A) a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 2 or a polypeptide consisting of an amino acid sequence that has at least 90% identity to the amino acid sequence shown in SEQ ID NO: 2 and has 4-hydroxybenzoate hydroxylase activity, (B) a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 6 or a polypeptide consisting of an amino acid sequence that has at least 90% identity to the amino acid sequence shown in SEQ ID NO: 6 and has 4-hydroxybenzoate hydroxylase activity.

Claims13

Dependent claims

A method for manufacturing a 3-hydroxy-4-aminobenzoic acid, comprising a step of bringing a 4-aminobenzoic acid into contact with a microorganism that produces polypeptide (A) or (B) shown below:

(A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:
2 or a polypeptide consisting of an amino acid sequence that has at least 90% identity to the amino acid sequence of SEQ ID NO:
2 and has 4-hydroxybenzoate hydroxylase activity, or
(B) a polypeptide consisting the an amino acid sequence of SEQ ID NO:
6 or a polypeptide consisting of an amino acid sequence that has at least 90% identity to the amino acid sequence of SEQ ID NO:
6 and has 4-hydroxybenzoate hydroxylase activity.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/269,689Filed 6 Sep 2019

Priority

JP JP2018-17184913 Sep 2018

Published

US 2021/0317484 A114 Oct 2021

Granted

26 Apr 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12P 13/001
Preparation of nitrogen-containing organic compounds: amines; Imines
C12P 13/005
Preparation of nitrogen-containing organic compounds: amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
C12N 9/0073
Acting on paired donors with incorporation of molecular oxygen: with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
C12N 15/09
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or…:…

Method for producing 3-hydroxy-4-aminobenzoic acid compound

Life of this patent

Method for producing 3-hydroxy-4-aminobenzoic acid compound

Life of this patent

Abstract

Claims13