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US 11,312,939 B2Granted
Constructs for chimeric antigen receptors
AssigneeCarisma Therapeutics Inc.
InventorsMichael Klichinsky, Nicholas G. Minutolo, Nicholas R. Anderson
Life of this patent
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Abstract
The present disclosure pertains to immune cells comprising chimeric antigen receptors (CARs) and methods of using immune cells comprising CARs.
Claims25
A method of modifying a macrophage or monocyte comprising delivering to the macrophage or monocyte:
- (i) a viral vector comprising one or more nucleic acid sequences encoding a chimeric antigen receptor (CAR) comprising:
- (a) an extracellular domain,
- (b) a transmembrane domain, and
- (c) an intracellular domain, and
- (ii) at least one Vpx protein,
- thereby producing a modified macrophage or monocyte that exhibits increased CAR expression relative to a macrophage or monocyte comprising a CAR delivered by a viral vector and not delivered at least one Vpx protein,
- wherein the modified macrophage or monocyte does not exhibit an M1 phenotype relative to a macrophage or monocyte not delivered at least one Vpx protein.
4 more elements
A method of modifying a macrophage or monocyte comprising delivering to the macrophage or monocyte:
- (i) a viral vector comprising one or more nucleic acid sequences encoding a chimeric antigen receptor (CAR) comprising:
- (a) an extracellular domain,
- (b) a transmembrane domain, and
- (c) an intracellular domain, and
- (ii) at least one Vpx protein, wherein the macrophage or monocyte is one or both of electroporated or transfected with at least one Vpx mRNA prior to transduction with the viral vector,
- thereby producing a modified macrophage or monocyte that exhibits increased CAR expression relative to a macrophage or monocyte comprising a CAR delivered by a viral vector and not delivered at least one Vpx protein.
3 more elements
Dependencies are inferred from the claim text.