Methods and systems to produce lignin-modifying enzymes and uses thereof
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsNikita A. Khlystov, Elizabeth Sattely
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Abstract
Embodiments of the invention are generally directed to lignin-modifying enzymes and systems and methods of their manufacture. In many embodiments, the lignin-modifying enzymes are lignin-degrading enzymes capable of breaking down lignin into component parts that are usable for other purposes. Several embodiments are directed to systems for producing lignin-modifying enzymes in vivo, including in yeast and/or plant species, and certain embodiments are directed to methods of creating these systems, including transfecting the species to produce lignin-modifying enzymes.
Claims20
A method to produce lignin-degrading enzymes comprising:
- obtaining an expression vector comprised of DNA and encoding a lignin-degrading peroxidase, a signal peptide to export the lignin-degrading peroxidase to an apoplast, and a constitutive promoter to drive transcription of the lignin-degrading peroxidase and the signal peptide, wherein the lignin-degrading peroxidase is encoded by a sequence selected from SEQ ID NO:
- 1, SEQ ID NO:
- 49, SEQ ID NO:
- 69, and SEQ ID NO:
- 74;
- transforming a plant with the expression vector;
- allowing the plant to express the lignin-degrading peroxidase contained within the expression vector by allowing the plant to grow for up to 15 days;
- and
- extracting the lignin-degrading peroxidase.
Dependencies are inferred from the claim text.