US 11,312,741 B2 · Separation of oligosaccharides from fermentation broth

Abstract

The invention relates to a method for obtaining an N-acetylglucosamine containing neutral oligosaccharide from a fermentation broth, wherein said oligosaccharide is produced by culturing a genetically modified microorganism capable of producing said oligosaccharide from an internalized carbohydrate precursor, comprising the steps of: i) ultrafiltration (UF), preferably to separate biomass from the broth, ii) nanofiltration (NF), preferably to concentrate said oligosaccharide in the broth and/or reduce an inorganic salt content of the broth, and iii) treating the broth with an ion exchange resin, preferably to remove charged materials, and/or subjecting the broth to chromatography, preferably to remove hydrophobic impurities.

Claims8

Dependent claims

A method for separating lacto-N-triose II, lacto-N-tetraose, or lacto-n-neotetraose from dissolved inorganic and organic salts, acids and bases in an aqueous medium from a fermentation or enzymatic process, comprising the ordered steps of:

applying ultrafiltration to said aqueous medium to form an ultrafiltration permeate;
applying nanofiltration to the ultrafiltration permeate to form a nanofiltrate retentate;
treating the nanofiltration retentate with a strong cation exchange resin in H+-form and then immediately with a weak anion exchange resin in free base form to form a low conductive solution of lacto-N-triose II, lacto-N-tetraose, or lacto-n-neotetraose, wherein the low conductive solution has a conductivity one to two orders of magnitude lower than the conductivity of said aqueous medium.

Dependencies are inferred from the claim text.

Separation of oligosaccharides from fermentation broth

Life of this patent

Separation of oligosaccharides from fermentation broth

Life of this patent

Abstract

Claims8