# US 11,307,203 B2 · Using phage epitopes to profile the immune response > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,307,203 B2 - Document ID: US11307203B2 - Status: Granted - Kind: B2 - Application number: 15/933,574 - Filed: 2018-03-23 - Published: 2018-08-09 - Granted: 2022-04-19 - Earliest claimed: 2010-03-17 - Assignees: The Regents of the University of Michigan; Exact Sciences Development Company, LLC - Applicants: The Regents of the University of Michigan; Exact Sciences Development Company, LLC - Inventors: Arul M. Chinnaiyan; Xiaoju Wang; Alex Tsodikov; Jeanne Ohrnberger - Web page: https://patentgrep.com/patent/US11307203B2 ## CPC - G01N 33/57555: of the prostate - C07K 16/00: Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies - C07K 16/005: {constructed by phage libraries} - G01N 33/57515: of the breast - G01N 33/5752: of the lungs - G01N 33/57535: of the large intestine, e.g. colon, rectum or anus - G01N 33/57585: {involving compounds identifiable in body fluids} - G01N 2333/4703: Regulators; Modulating activity - G01N 2333/4704: Inhibitors; Supressors - G01N 2333/705: Assays involving receptors, cell surface antigens or cell surface determinants - G01N 2333/912: transferring phosphorus containing groups, e.g. kinases (2.7) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract The present disclosure provides compositions and methods for using one or more polypeptide probes to profile an immune response. The polypeptide probe can be used to detect one or more antibodies from a sample. Furthermore, the present disclosure provides methods and compositions for characterizing a cancer based on the detection of one or more antibodies, such as autoantibodies. ## Claims ### Claim 1 A kit, comprising: a plurality of polypeptide probes, wherein said polypeptide probes comprise all of: (i) amino acid sequence SEQ ID NO: 13 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 27, (ii) amino acid sequence SEQ ID NO: 132 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 162, (iii) amino acid sequence SEQ ID NO: 2 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 16, (iv) amino acid sequence SEQ ID NO: 141 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 171, (v) amino acid sequence SEQ ID NO: 129 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 159, (vi) amino acid sequence SEQ ID NO: 118 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 148, (vii) amino acid sequence SEQ ID NO: 122 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 152, and (viii) amino acid sequence SEQ ID NO: 130 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 160. ### Claim 2 (depends on 1) The kit of claim 1, wherein said kit further comprises one or more polypeptide probes comprising a polypeptide or epitope fragment of amino acid sequence SEQ ID NO: 5, 11, 12, 14, 56, 58, 60, 62, 64, 67, 69, 115, 116, 123 or 140. ### Claim 3 (depends on 1) The kit of claim 1, wherein said kit further comprises one or more polypeptide probes comprising a polypeptide or epitope fragment of amino acid sequence SEQ ID NO: 19, 25, 26, 28, 70, 73, 75, 77, 79, 82, 84, 145, 146, 153, or 170. ### Claim 4 A kit, comprising: a plurality of polypeptide probes attached to a substrate, wherein said polypeptide probes comprise all of: (i) amino acid sequence SEQ ID NO: 13 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 27, (ii) amino acid sequence SEQ ID NO: 132 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 162, (iii) amino acid sequence SEQ ID NO: 2 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 16, (iv) amino acid sequence SEQ ID NO: 141 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 171, (v) amino acid sequence SEQ ID NO: 129 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 159, (vi) amino acid sequence SEQ ID NO: 118 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 148, (vii) amino acid sequence SEQ ID NO: 122 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 152, and (viii) amino acid sequence SEQ ID NO: 130 or an amino acid sequence encoded by nucleic acid sequence SEQ ID NO: 160. ### Claim 5 (depends on 4) The kit of claim 4, wherein said kit further comprises one or more polypeptide probes comprising a polypeptide or epitope fragment of amino acid sequence SEQ ID NO: 5, 11, 12, 14, 56, 58, 60, 62, 64, 67, 69, 115, 116, 123 or 140. ### Claim 6 (depends on 4) The kit of claim 4, wherein said kit further comprises one or more polypeptide probes comprising a polypeptide or epitope fragment of amino acid sequence SEQ ID NO: 19, 25, 26, 28, 70, 73, 75, 77, 79, 82, 84, 145, 146, 153, or 170. ### Claim 7 (depends on 4) The kit of claim 4, wherein said substrate is an array, bead, or particle.