# US 11,307,201 B2 · Self-actuating signal producing detection devices and methods

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,307,201 B2
- Document ID: US11307201B2
- Status: Granted
- Kind: B2
- Application number: 12/211,992
- Filed: 2008-09-17
- Published: 2009-05-07
- Granted: 2022-04-19
- Earliest claimed: 2007-09-17
- Assignees: Red Ivory LLC
- Applicants: Marc Robert Labgold; George G Jokhadze
- Inventors: Marc Robert Labgold; George G Jokhadze
- Web page: https://patentgrep.com/patent/US11307201B2

## CPC

- G01N 33/5438: {Electrodes}
- C12Q 1/6825: Nucleic acid detection involving sensors
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

An assay system is provided of great sensitivity and portability where the presence of a specific target in a sample, as well as its concentration (qualification and quantification) is detected by reason of a potential or voltage in a closed circuit, built up a redox reaction. The reaction is produced by binding a capture moiety to an enzymatic redox reaction partner, allowing the capture moiety to bind to any target in the sample, and washing any such bound target. The bound target, if not immobilized, may be immobilized through use of a second capture moiety. Substrate for the enzyme is then added. The action of the enzyme upon the substrate frees electrons, creating a potential across an anode and cathode which may be separated by a membrane.

## Claims

### Claim 1

An assay system for the detection of a target in a liquid sample, wherein said assay system comprises:
a first capture moiety which binds to any target present in said sample, said first capture moiety complexed with a redox reaction enzyme;
a substrate recognized by said enzyme, which substrate, when acted upon by said enzyme, releases electrons; and
an electronic circuit for self-actuating signal producing detection of electric potential or current developed by the release of electrons from said substrate when digested by said enzyme, wherein said electric potential or current is detected in the absence of any source of electrical power provided to said system other than the electrical power generated by the release of electrons from said substrate when digested by said enzyme.

### Claim 2 (depends on 1)

The assay system of claim 1, wherein said circuit detects the presence of said electric potential or current directly.

### Claim 3 (depends on 1)

The assay system of claim 1, wherein said circuit specifically detects directly said released electrons.