# US 11,307,199 B2 · Oxidase activity of polymeric coated cerium oxide nano-particles

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- Number: US 11,307,199 B2
- Document ID: US11307199B2
- Status: Granted
- Kind: B2
- Application number: 16/358,049
- Filed: 2019-03-19
- Published: 2019-09-12
- Granted: 2022-04-19
- Earliest claimed: 2009-03-17
- Assignees: University of Central Florida Research Foundation, Inc.
- Applicants: University of Central Florida Research Foundation, Inc.
- Inventors: Jesus Manuel Perez; Atul Asati; Santimukul Santra; Charalambos Kaittanis; Sudip Nath
- Web page: https://patentgrep.com/patent/US11307199B2

## CPC

- G01N 33/54346: {Nanoparticles}
- B01J 31/26: containing in addition, inorganic metal compounds not provided for in groups B01J31/02 - B01J31/24
- B82Y 15/00: Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
- G01N 33/5432: {Liposomes or microcapsules}
- G01N 33/5752: of the lungs
- G01N 33/582: {with fluorescent label}
- G01N 33/587: {Nanoparticles}
- G01N 33/9413: {Dopamine}
- B01J 2231/005: General concepts, e.g. reviews, relating to methods of using catalyst systems, the concept being defined by a common method or theory, e.g. microwave heating or multiple stereoselectivity
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES

## Abstract

Methods, systems, compositions include biocompatible polymer coated nanoceria that function as aqueous redox catalyst with enhanced activity at an acidic to moderately alkaline pH value between 1 and 8. The compositions are used as oxidizing agents for decomposition, decontamination or inactivation of organic contaminants, such as, pesticides and chemical warfare agents. Another use includes nanoceria as targetable nanocatalyst prepared by conjugating various targeting ligands to the nanoparticle coating to form a colorimetric or fluorescent probe in immunoassays and other molecule binding assays that involve the use of a molecule in solution that changes the color of the solution or emits a fluorescent signal, where localization of nanoceria to organs or tissue is assessed by treatment with an oxidation sensitive dye or other detection devices. Versatility and uses of the nanoceria compositions are controlled by pH value, choice of dye substrate and thickness of the polymer coating on the ceria nanoparticles.

## Claims

### Claim 1

A method of conducting an oxidase reaction using a plurality of biocompatible polymer coated ceria nanoparticles, the method comprising:
preparing an aqueous solution in which at least one substrate selected from the group consisting of 10-acetyl-3,7-dihydroxyphenoxazine and orthophenylene diamine (OPD), and the plurality of biocompatible polymer coated ceria nanoparticles are contained;
wherein the pH of the aqueous solution containing the substrate and the plurality of biocompatible polymer coated ceria nanoparticles is between approximately 4.0 and approximately 8.0.

### Claim 2 (depends on 1)

The method of claim 1, wherein the biocompatible polymer coating on the biocompatible polymer coated nanoparticles comprising Protein-G or Protein-A conjugated thereon.

### Claim 3 (depends on 2)

The method of claim 2, wherein the Protein-G or Protein-A comprises an antibody bound thereto.