# US 11,307,198 B2 · Compositions and methods for antigen detection incorporating inorganic nanostructures to amplify detection signals

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- Number: US 11,307,198 B2
- Document ID: US11307198B2
- Status: Granted
- Kind: B2
- Application number: 15/495,852
- Filed: 2017-04-24
- Published: 2017-11-23
- Granted: 2022-04-19
- Earliest claimed: 2016-04-26
- Assignees: Washington State University
- Applicants: Washington State University
- Inventors: Yuehe Lin; Dan Du; Yang Song; Ranfeng Ye
- Web page: https://patentgrep.com/patent/US11307198B2

## CPC

- G01N 33/54346: {Nanoparticles}
- G01N 33/553: Metal or metal coated
- G01N 33/581: {with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)}
- G01N 33/587: {Nanoparticles}
- C12Q 1/00: Measuring or testing processes involving enzymes, nucleic acids or microorganisms (measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34); Compositions therefor; Processes of preparing such compositions
- G01N 33/00: Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The disclosure relates to antigen detection reagents and related methods, systems, and kits. The reagents comprise an antigen-binding molecule conjugated to an inorganic component. In some embodiments, the inorganic component possesses catalytic functionality to provide a detectable signal. In some embodiments, the catalytic inorganic component is or comprises a bimetallic nanoparticle. In other embodiments, the inorganic component is a nanoflowers that provides a physical scaffold onto which the antigen-binding component and a reporter component can be loaded, resulting in augmented antigen-binding and reporting capabilities.

## Claims

### Claim 1

An antigen detection reagent, comprising an antigen-binding molecule and a reporter component conjugated to an inorganic nanoflower component,
wherein:
the inorganic nanoflower component comprises Cu3(PO4)2, Mn3(PO4)2, or CaHPO4;
the reporter component is a reporter enzyme; and
the reporter component is configured to cooperate with the antigen-binding molecule to provide an amplified signal.

### Claim 2 (depends on 1)

The antigen detection reagent of claim 1, wherein the reporter enzyme is horseradish peroxidase (HRP), invertase, glucose oxidase (GOx), or alkaline phosphatase (AP).