Polyarginine-coated magnetic nanovector and methods of use thereof
AssigneeUniversity of Washington
InventorsMiqin Zhang, Omid Veiseh, Chen Fang, Forrest Kievit
Life of this patent
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Abstract
Polyarginine-coated nanoparticle, and methods for making and using the nanoparticle. The nanoparticle can have a core that includes a material that imparts magnetic resonance imaging activity to the particle and, optionally, include one or more of an associated therapeutic agent, targeting agent, and diagnostic agent.
Claims13
A method for silencing or reducing the expression level of a gene, comprising contacting a cancer cell of interest with a nanovector effective to silence or reduce the expression level of the gene, which comprises a nanoparticle and a therapeutic agent, wherein the nanoparticle comprises:
- (a) a core consisting of a core material and a core surface, wherein the core material is a material having magnetic resonance imaging activity;
- (b) a polyarginine coating covalently coupled to the core surface;
- and
- (c) a poly(alkylene oxide) oligomer intermediate the core surface and the polyarginine coating, to which the polyarginine coating is covalently bonded.
A method for silencing or reducing the expression level of a gene, comprising contacting a cancer cell of interest with a nanovector effective to silence or reduce the expression level of the gene, which comprises a nanoparticle and a therapeutic agent, wherein the nanoparticle comprises:
- (a) a core consisting of a core material and a core surface;
- (b) a polyarginine coating covalently coupled to the core surface;
- and
- (c) a poly(alkylene oxide) oligomer intermediate the core surface and the polyarginine coating, to which the polyarginine coating is covalently bonded;
- and
- (d) a fluorescent agent covalently attached to the nanoparticle.
A method for silencing or reducing the expression level of a gene, comprising contacting a cancer cell of interest with a nanovector effective to silence or reduce the expression level of the gene, which comprises a nanoparticle and a therapeutic agent, wherein the nanoparticle comprises:
- (a) a core consisting of a core material and a core surface;
- (b) a polyarginine coating covalently coupled to the core surface;
- (c) a poly(alkylene oxide) oligomer intermediate the core surface and the polyarginine coating, to which the polyarginine coating is covalently bonded;
- (d) a fluorescent agent covalently attached to the nanoparticle;
- and
- (e) a targeting agent.
Dependencies are inferred from the claim text.