# US 11,307,184 B2 · Sensor arrays with nucleophilic indicators > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,307,184 B2 - Document ID: US11307184B2 - Status: Granted - Kind: B2 - Application number: 15/897,403 - Filed: 2018-02-15 - Published: 2018-08-16 - Granted: 2022-04-19 - Earliest claimed: 2017-02-16 - Assignees: iSense Corporation - Applicants: iSense LLC - Inventors: Sung Hyun Lim; Autumn Maruniak; Nuria Queralto Gratacos - Web page: https://patentgrep.com/patent/US11307184B2 ## CPC - G01N 31/22: using chemical indicators (G01N31/02 takes precedence) - G01N 21/78: producing a change of colour - G01N 31/223: {for investigating presence of specific gases or aerosols (G01N31/221, G01N31/222 take precedence; actuation of fire alarm by presence of smoke or gases G08B17/10)} - G01N 33/497: of gaseous biological material, e.g. breath - G01N 33/56911: {Bacteria} - G01N 33/575: for cancer - G01N 33/57535: of the large intestine, e.g. colon, rectum or anus - G01N 33/0031: {comprising two or more sensors, e.g. a sensor array} - G01N 2021/7793: {Sensor comprising plural indicators} - G01N 2430/00: Assays, e.g. immunoassays or enzyme assays, involving synthetic organic compounds as analytes - G01N 2800/26: Infectious diseases, e.g. generalised sepsis - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Sensors arrays that include indicators arranged in a pattern on a substrate. The indicators include nucleophilic indicators. In some examples, the sensor arrays with multiple nucleophilic indicators provide superior detection and identification of microorganisms, cancer biomarkers, formaldehyde, organophosphates, and aldehydes. ## Claims ### Claim 1 A sensor array comprising: a substrate; a first nucleophilic indicator deposited on the substrate, the first nucleophilic indicator including a parosoaniline colorant; and a second nucleophilic indicator deposited on the substrate, the second nucleophilic indicator including an N-(rhodamine B)-deoxylactam-ethylenediamine colorant, wherein the first nucleophilic indicator and the second nucleophilic indicator are deposited at separate locations on the substrate in a predetermined pattern, each of the first nucleophilic indicator and the second nucleophilic indicator has a distinct spectral response to at least one distinct analyte, wherein a computer is configured to: capture a color change of the first nucleophilic indicator over a time period and a color change of the second nucleophilic indicator over the time period, in response to the at least one distinct analyte coming in contact with both the parosoaniline colorant and the N-(rhodamine B)-deoxylactam-ethylenediamine colorant, and compare the captured color change of the first nucleophilic indicator and the captured color change of the second nucleophilic indicator over the time period to a stored library of color response patterns to determine concentration of the at least one distinct analyte. ### Claim 2 (depends on 1) The sensor of claim 1, wherein the at least one distinct analyte is in a solution. ### Claim 3 (depends on 1) The sensor of claim 1, wherein the at least one distinct analyte is a volatile organic compound or mixtures of volatile organic compounds. ### Claim 4 (depends on 1) The sensor of claim 1, wherein the at least one distinct analyte is a cancer biomarker. ### Claim 5 (depends on 1) The sensor of claim 1, wherein the at least one distinct analyte is an aldehyde. ### Claim 6 (depends on 1) The sensor of claim 1, wherein the at least one distinct analyte is an organophosphate. ### Claim 7 (depends on 1) The sensor of claim 1, wherein the at least one distinct analyte is an electrophilic analyte. ### Claim 8 (depends on 1) The sensor of claim 1, wherein the at least one distinct analyte is a ketone. ### Claim 9 (depends on 1) The sensor of claim 1, wherein the distinct spectral response is in an ultraviolet range. ### Claim 10 (depends on 1) The sensor of claim 1, wherein a nucleophilic dye of the first nucleophilic indicator and the second nucleophilic indicator comprises at least one of the following: a plasticizer, a sol-gel, or a polymer. ### Claim 11 A method for detecting an analyte comprising: providing a sensor array comprising a first nucleophilic indicator and a second nucleophilic indicator deposited at separate locations on a substrate in a predetermined pattern, the first and second nucleophilic indicators having a distinct spectral response to at least one distinct analyte; exposing the sensor array to the at least one analyte; capturing, by a computer, a color change of the first nucleophilic indicator over a time period and a color change of the second nucleophilic indicator over the time period, in response to the at least one distinct analyte coming in contact with both a parosoaniline colorant in the first nucleophilic indicator and a N-(rhodamine B)-deoxylactam-ethylenediamine colorant in the second nucleophilic indicator; and comparing, by the computer, the captured color change of the first nucleophilic indicator and the captured color change of the second nucleophilic indicator over the time period to a stored library of color response patterns to determine a concentration of the at least one distinct analyte. ### Claim 12 (depends on 11) The method of claim 11, wherein the analyte is a volatile organic compound. ### Claim 13 (depends on 11) The method of claim 11, wherein the analyte is in a solution when it is exposed to the sensor array. ### Claim 14 (depends on 11) The method of claim 11, wherein the spectral response is in an ultraviolet range. ### Claim 15 (depends on 11) The method of claim 11, wherein exposing the sensor array to the analyte comprises exposing the sensor array to a gas that contains the analyte. ### Claim 16 (depends on 15) The method of claim 15, wherein the analyte comprises a volatile organic compound. ### Claim 17 (depends on 11) The method of claim 11, wherein exposing the sensor array to the analyte comprises exposing the sensor array to a liquid that contains the analyte. ### Claim 18 (depends on 1) The sensor array of claim 1 wherein the at least one distinct analyte is formaldehyde.