# US 11,307,165 B2 · Electrochemical sensors with a chemically attached molecular redox buffer

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- Number: US 11,307,165 B2
- Document ID: US11307165B2
- Status: Granted
- Kind: B2
- Application number: 15/655,457
- Filed: 2017-07-20
- Published: 2018-01-25
- Granted: 2022-04-19
- Earliest claimed: 2016-07-21
- Assignees: Regents of the University of Minnesota
- Applicants: Regents of the University of Minnesota
- Inventors: Jinbo Hu; Philippe Buhlmann; Andreas Stein; Xue Zhen
- Web page: https://patentgrep.com/patent/US11307165B2

## CPC

- G01N 27/333: Ion-selective electrodes or membranes (glass electrodes G01N27/36)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Solid-contact electrodes include a molecular redox buffer that is covalently attached to one of the components of the electrodes. These electrodes can be incorporated into electrochemical sensor systems. The electrode components are an electron conductor, a membrane and optionally, an interlayer. The electrode includes covalent attachment of the molecular redox buffer to the interlayer, to the electron conductor or the membrane. The interlayer includes nanoporous carbon such as CIM-carbon. The molecular redox buffer includes a well-defined redox pair, e.g., a complex of Co(II)/Co(III). The electrodes are ion-selective electrodes (ISEs) or reference electrodes. Methods include constructing solid contact electrodes and using the electrodes in electrochemical sensor systems to determine the quantity of an analyte in a sample. The electrochemical sensor systems include disposable paper-based devices and/or wearable devices for measuring analytes in a sample.

## Claims

### Claim 1

A solid-contact (SC)-electrode comprising a solid electron conductor, an interlayer, a membrane and a molecular redox buffer, wherein the interlayer comprises nanoporous carbon and is located between the solid electron conductor and the membrane, wherein the nanoporous carbon is modified by a reactive functional group selected from the group consisting of azide (—N3), thiol (—SH), trichlorosilane, and alkoxysilane, wherein the molecular redox buffer comprises a metal ion redox couple, the metal ion redox couple comprising a reduced species and an oxidized species, wherein the reduced species and the oxidized species of the molecular redox buffer are covalently attached to the nanoporous carbon in the interlayer through the reactive functional group.

### Claim 2 (depends on 1)

The SC-electrode of claim 1, wherein the nanoporous carbon is colloid-imprinted mesoporous (CIM) carbon.

### Claim 3 (depends on 2)

The SC-electrode of claim 2, wherein the CIM carbon is soaked and electrochemically modified in the presence of a diazonium compound.

### Claim 4 (depends on 1)

The SC-electrode of claim 1, wherein the molecular redox buffer comprises complexes with pyridines, bipyridines, terpyridines, porphyrins, phthalocyanines, phenanthrolines, acetylacetones, bipyridylimino isoindolines, crown ethers, cryptands and combinations thereof.

### Claim 5 (depends on 1)

The SC-electrode of claim 1, wherein the metal ion redox couple is selected from Co2+/Co3+, Os2+/Os3+, Fe2+/Fe3+, Cu+/Cu2+, Ni2+/Ni3+, Mn2+/Mn3+, Cr2+/Cr3+, Ru2+/Ru3+, Mo4+/Mo5+, Ag+/Ag2+, Ag2+/Ag3+, Sn2+/Sn4+, Pb2+/Pb4+ and Ir2+/Ir3+.

### Claim 6 (depends on 1)

The SC-electrode of claim 1, wherein the linkages formed between the reactive functional group and the molecular redox buffer are ester linkages, amide linkages, carbon-carbon linkages or carbon-silicon linkages.

### Claim 7 (depends on 1)

The SC-electrode of claim 1, wherein the solid electron conductor is selected from gold, platinum, silver, copper, steel, carbon, nickel, brass, indium-tin-oxide (ITO), fluorine-doped tin oxide (FTO), aluminum and combinations thereof.

### Claim 8 (depends on 1)

The SC-electrode of claim 1, wherein the molecular redox buffer is synthesized as a diazonium salt.

### Claim 9 (depends on 1)

The SC-electrode of claim 1, wherein the electrode is incorporated within an electrochemical sensor system comprising two or more electrodes.

### Claim 10 (depends on 9)

The SC-electrode of claim 9, wherein the electrochemical sensor system measures analytes selected from H+, Li+, Na+, K+, Mg2+, Ca2+, Cu2+, Ag+, Zn2+, Cd2+, Hg2+, Pb2+, NH4+, carbonate, bicarbonate, nitrate, nitrite, sulfide, phosphate, chloride, iodide, perfluoroalkylsulfonates, perfluoroalkanoates and combinations thereof.

### Claim 11 (depends on 9)

The SC-electrode of claim 9, wherein at least one of the electrodes in the electrochemical sensor system is a solid-contact reference electrode, and wherein the membrane in the reference electrode is a reference membrane.

### Claim 12 (depends on 9)

The SC-electrode of claim 9, wherein at least one of the electrodes is a solid-contact ion-selective electrode, and wherein the membrane in the ion-selective electrode is an ion-selective membrane.

### Claim 13 (depends on 9)

The SC-electrode of claim 9, wherein the system is a wearable system, an implantable system, a printed system, a paper-based system or combinations thereof.

### Claim 14

A method of making an electrochemical sensor system, the method comprising:
forming a solid-contact (SC)-electrode comprising a solid electron conductor, an interlayer, a membrane and a molecular redox buffer, wherein the interlayer comprises nanoporous carbon and is located between the solid electron conductor and the membrane, wherein the nanoporous carbon is modified by a reactive functional group selected from the group consisting of azide (—N3), thiol (—SH), trichlorosilane, and alkoxysilane, wherein the molecular redox buffer comprises a metal ion redox couple, the metal ion redox couple comprising a reduced species and an oxidized species and wherein the reduced species and the oxidized species of the molecular redox buffer are covalently attached to the nanoporous carbon in the interlayer through the reactive functional group.

### Claim 15 (depends on 14)

The method of claim 14, wherein the nanoporous carbon interlayer is the colloid-imprinted mesoporous (CIM) carbon interlayer.

### Claim 16 (depends on 14)

The method of claim 14, wherein the metal ion redox couple is selected from Co2+/Co3+, Os2+/Os3+, Fe2+/Fe3+, Cu+/Cu2+, Ni2+/Ni3+, Mn2+/Mn3+, Cr2+/Cr3+, Ru2+/Ru3+, Mo4+/Mo5+, Ag+/Ag2+, Ag2+/Ag3+, Sn2+/Sn4+, Pb2+/Pb4+ and Ir2+/Ir3+.

### Claim 17 (depends on 14)

The method of claim 14, wherein linkages formed between the reactive functional group and the molecular redox buffer are ester linkages, amide linkages, carbon-carbon linkages or carbon-silicon linkages.

### Claim 18 (depends on 14)

The method of claim 14, wherein the molecular redox buffer complex is synthesized prior to coupling to the conductor, the interlayer or the membrane.

### Claim 19 (depends on 14)

The method of claim 14, wherein the molecular redox buffer is synthesized on the interlayer with a reactive functional group from reactant monomers.

### Claim 20

A method of measuring an analyte in a sample comprising:
contacting an ion-selective electrode in an electrochemical system with a sample, the electrochemical system comprising at least one solid-contact (SC)-electrode, wherein the SC-electrode comprises a solid electron conductor, an interlayer, a membrane and a molecular redox buffer, wherein the interlayer comprises nanoporous carbon and is between the conductor and the membrane, wherein the nanoporous carbon is modified by a reactive functional group selected from the group consisting of azide (—N3), thiol (—SH), trichlorosilane, and alkoxysilane, wherein the molecular redox buffer comprises a metal ion redox couple, the metal ion redox couple comprising a reduced species and an oxidized species and wherein the reduced species and the oxidized species of the molecular redox buffer are covalently attached to the nanoporous carbon in the interlayer through the reactive functional group.

### Claim 21 (depends on 20)

The method of claim 20, wherein the electrochemical sensor system comprises at least two SC-electrodes, and wherein at least one of the SC-electrodes comprises the ion-selective electrode or the reference electrodes.

### Claim 22 (depends on 20)

The method of claim 20, wherein the sample is a clinical sample, an industrial sample, a forensic sample, an agricultural sample, a residential sample, a bodily fluid sample, or an environmental sample.

### Claim 23 (depends on 20)

The method of claim 20, wherein the electrochemical system comprises a sensor, wherein the sensor is a potentiometric sensor, ion-sensitive field effect transistor, a voltammetric sensor, an amperometric sensor, a coulometric sensor, or an impedance sensor.

### Claim 24 (depends on 20)

The method of claim 20, wherein the method further comprises correlating the results from the sensor to determine the quantity or concentration of the analyte.

### Claim 25 (depends on 20)

The method of claim 20, wherein the analyte measured is selected from H+, Li+, Na+, K+, Mg2+, Ca2+, Cu2+, Ag+, Zn2+, Cd2+, Hg2+, Pb2+, NH4+, carbonate, bicarbonate, nitrate, nitrite, sulfide, phosphate, chloride, iodide, perfluoroalkylsulfonates, perfluoroalkanoates and combinations thereof.