# US 11,307,146 B2 · Systems and methods for detection of chemtiluminescent reactions

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- Number: US 11,307,146 B2
- Document ID: US11307146B2
- Status: Granted
- Kind: B2
- Application number: 15/661,696
- Filed: 2017-07-27
- Published: 2018-02-01
- Granted: 2022-04-19
- Earliest claimed: 2016-07-29
- Assignees: University of Houston System; Instituto Tecnologico Y De Estudios Superiores De Monterrey
- Applicants: University of Houston System; Instituto Tecnológico y de Estudios Superiores de Monterrey
- Inventors: Richard C. Willson; Jinsu Kim; Binh V. Vu; Olga Patricia Vázquez Villegas; Federico Augusto Ruiz Ruiz; Marco Antonio Rito Palomares
- Web page: https://patentgrep.com/patent/US11307146B2

## CPC

- G01N 21/76: Chemiluminescence; Bioluminescence
- C12Q 1/26: involving oxidoreductase
- G01N 33/62: involving urea
- G01N 33/66: involving blood sugars, e.g. galactose
- G01N 33/70: involving creatine or creatinine
- G01N 2021/755: {Comparing readings with/without reagents, or before/after reaction}
- G01N 2800/042: Disorders of carbohydrate metabolism, e.g. diabetes, glucose metabolism
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Methods, devices and kit for analyzing a sample comprising 1,5-anhydroglucitol and a first analyte via one or more chemiluminescent reactions. Certain embodiments include measuring a first light response resulting from a first chemiluminescent reaction and measuring a second light response resulting from a second chemiluminescent reaction. Certain embodiments also include comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first analyte.

## Claims

### Claim 1

A method for analyzing a sample, the method comprising:
(a) obtaining a sample comprising 1,5-anhydroglucitol and a first analyte;
(b) adding glucose oxidase to the sample, wherein the glucose oxidase causes a first chemiluminescent reaction with the sample;
(c) measuring photons from a first light response resulting from the first chemiluminescent reaction;
(d) adding pyranose oxidase to the sample, wherein:
the pyranose oxidase is sequentially added to the sample after glucose oxidase is added to the sample; and
the pyranose oxidase causes a second chemiluminescent reaction with the sample;
(e) measuring photons from a second light response resulting from the second chemiluminescent reaction; and
(f) calculating a ratio of 1,5-anhydroglucitol and the first analyte based on the measured photons from the first light response to the photons from the second light response, wherein:
measuring photons from the first light response resulting from the first chemiluminescent reaction and measuring photons from the second light response resulting from the second chemiluminescent reaction comprises measuring photons with a light detector; and
comparing the photons from the first light response to the photons from the second light response to calculate a ratio of 1,5-anhydroglucitol and the first analyte comprises transmitting data to a computer processor.

### Claim 2 (depends on 1)

The method of claim 1 wherein the sample comprises saliva.

### Claim 3 (depends on 1)

The method of claim 1 wherein the sample comprises urine.

### Claim 4 (depends on 1)

The method of claim 1 wherein the sample comprises blood.

### Claim 5 (depends on 1)

The method of claim 1 wherein the sample comprises interstitial fluid.

### Claim 6 (depends on 1)

The method of claim 1 wherein the first analyte is glucose, L-sorbose, D-xylose, D-galactose, glucono-δ-lactone, urea, creatinine, or creatine.

### Claim 7 (depends on 1)

The method of claim 1 wherein the light detector is selected from the group consisting of a charged coupled device (CCD), avalanche diode, multi-pixel photon counter (MPPC), silicon photomultiplier (SiPMT), complementary metal-oxide-semiconductor (CMOS) sensor, scientific CMOS (sCMOS) sensor, photomultiplier tube (PMT), photodiode, camera, cellular phone camera, web camera, and smart watch camera.

### Claim 8 (depends on 1)

The method of claim 1 wherein the glucose oxidase and the pyranose oxidase are added to the sample via a microfluidic device.

### Claim 9 (depends on 1)

The method of claim 1 further comprising accessing a lookup table with the computer processor.

### Claim 10 (depends on 9)

The method of claim 9 wherein the lookup table comprises an indication of a physiological condition.

### Claim 11 (depends on 10)

The method of claim 10 wherein the physiological condition is related to an insulin level of a person from whom the sample was obtained.

### Claim 12 (depends on 1)

The method of claim 1 further comprising normalizing the ratio based on a measurement of a marker in the sample.

### Claim 13 (depends on 12)

The method of claim 12 wherein the marker is urea, creatinine, creatine, human serum albumin, or hemoglobin.

### Claim 14 (depends on 13)

The method of claim 13 wherein the sample comprises urine, blood or saliva.