# US 11,307,095 B2 · Systems and methods for in situ optimization of tunable light emitting diode sources > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,307,095 B2 - Document ID: US11307095B2 - Status: Granted - Kind: B2 - Application number: 17/112,393 - Filed: 2020-12-04 - Published: 2021-06-10 - Granted: 2022-04-19 - Earliest claimed: 2019-12-04 - Assignees: ChemImage Corporation - Applicants: Chemimage Corporation - Inventors: Shawna Tazik; Alyssa Zrimsek; Heather Gomer; Jihang Wang; Patrick J. Treado - Web page: https://patentgrep.com/patent/US11307095B2 ## CPC - G01J 3/2823: {Imaging spectrometer} - G01J 3/10: Arrangements of light sources specially adapted for spectrometry or colorimetry - G01J 3/12: Generating the spectrum; Monochromators - G01J 3/2803: {using photoelectric array detector} - G01J 3/42: Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry (beam switching arrangements G01J3/08) - G01J 2003/1282: {Spectrum tailoring} - G01J 2003/2826: {Multispectral imaging, e.g. filter imaging} - G01J 2003/283: {computer-interfaced} - G01J: MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY (light sources F21, H01J, H01K, H05B; investigating properties of materials by optical means G01N) ## Abstract Systems for and methods for in situ optimization of tunable light emitting diode sources are disclosed herein. During operation, the systems and methods obtain real-time feedback from an image sensor, and that feedback is used to tune the tunable LEDs. By tuning the tunable LEDs, the best values for the LED spectral output can be selected based on the feedback from the image sensor, and an image with improved contrast is obtained. Alternatively, the amount of time to obtain an image with acceptable contrast is reduced. ## Claims ### Claim 1 A system comprising: a processor; and a non-transitory processor-readable storage medium in operable communication with the processor, wherein the storage medium contains one or more programming instructions that, when executed, cause the processor to perform the following: receiving at least a first data set and a second data set from an image sensor, the first and second data sets being collected by the image sensor based on illumination of a sample using a tunable light emitting diode source having a plurality of tuning states corresponding to a plurality of illumination spectrums, wherein the first data set and the second data set are associated with a first tuning state associated with a first illumination spectrum and a second tuning state associated with a second illumination spectrum of the tunable light emitting diode source, respectively; generating a score image data set based on an optical computation applied to the first and second data sets; determining whether the score image data set satisfies a tolerance level; and generating a set of test data based on the score image data set when the score image data set satisfies the tolerance level. ### Claim 2 (depends on 1) The system of claim 1, wherein the storage medium contains at least one additional programming instruction that, when executed, cause the processor to perform the following: analyzing the set of test data to determine at least one characteristic of the sample. ### Claim 3 (depends on 2) The system of claim 2, wherein the at least one characteristic comprises one or more of a presence of an analyte in the sample, an absence of the analyte in the sample, a classification of the analyte, a non-classification of the analyte, or a concentration of the analyte. ### Claim 4 (depends on 1) The system of claim 1, wherein the first data set is representative of a first set of interacted photons based on the first illumination spectrum and the second data set is representative of a second set of interacted photons based on the second illumination spectrum. ### Claim 5 (depends on 4) The system of claim 4, wherein the first set of interacted photons and the second set of interacted photons comprise one or more of photons absorbed by the sample, photons reflected from the sample, photons scattered by the sample, photons emitted by the sample, or photons transmitted through the samples. ### Claim 6 (depends on 1) The system of claim 1, wherein the set of test data provides visual contrast between the sample and background materials. ### Claim 7 (depends on 1) The system of claim 1, wherein the storage medium contains at least one additional programming instruction that, when executed, cause the processor to perform the following: repeating the receiving, generating, and determining steps for at least one additional tuning state in response to the score image set of data not satisfying the tolerance level. ### Claim 8 (depends on 7) The system of claim 7, wherein the repeating is performed until the tolerance level is satisfied. ### Claim 9 (depends on 7) The system of claim 7, wherein the repeating is performed a pre-determined number of times or for a pre-determined amount of time. ### Claim 10 (depends on 1) The system of claim 1, wherein the first data set comprises a first image (T1) and the second data set comprises a second image (T2), and the optical computation comprises one or more of T1+T2, T1−T2, T1*T2, T1/T2, or combinations thereof. ### Claim 11 (depends on 1) The system of claim 1, wherein the tolerance level comprises at least one figure of merit. ### Claim 12 (depends on 1) The system of claim 1, wherein the at least one figure of merit comprises one or more of an area under the receiver operator characteristic (AUROC) curve, standard error of calibration (SEC), signal to noise ratio (SNR), a Fisher contrast ratio, standard error of prediction (SEP), or optical throughput (% T). ### Claim 13 (depends on 1) The system of claim 1, wherein the tunable light emitting diode has a plurality of channels corresponding to a band of light and each combination of the plurality of channels corresponds to one of the plurality of tuning states. ### Claim 14 A system comprising: a tunable light emitting diode source having a plurality of tuning states corresponding to a plurality of illumination spectrums; an image sensor; and a computing device comprising: a processor; and a non-transitory processor-readable storage medium in operable communication with the processor, wherein the storage medium contains one or more programming instructions that, when executed, cause the processor to perform the following: receiving at least a first data set and a second data set from the image sensor, the first and second data sets being collected by the image sensor based on illumination of a sample using, wherein the first data set and the second data set are associated with a first tuning state associated with a first illumination spectrum and a second tuning state associated with a second illumination spectrum of the tunable light emitting diode source, respectively; generating a score image data set based on an optical computation applied to the first and second data sets; determining whether the score image data set satisfies a tolerance level; and generating a set of test data based on the score image data set when the score image data set satisfies the tolerance level. ### Claim 15 (depends on 14) The system of claim 14, wherein the storage medium contains at least one additional programming instruction that, when executed, cause the processor to perform the following: analyzing the set of test data to determine at least one characteristic of the sample. ### Claim 16 (depends on 15) The system of claim 15, wherein the at least one characteristic comprises one or more of a presence of an analyte in the sample, an absence of the analyte in the sample, a classification of the analyte, a non-classification of the analyte, or a concentration of the analyte. ### Claim 17 (depends on 14) The system of claim 14, wherein the first data set is representative of a first set of interacted photons based on the first illumination spectrum and the second data set is representative of a second set of interacted photons based on the second illumination spectrum. ### Claim 18 (depends on 17) The system of claim 17, wherein the first set of interacted photons and the second set of interacted photons comprise one or more of photons absorbed by the sample, photons reflected from the sample, photons scattered by the sample, photons emitted by the sample, or photons transmitted through the sample. ### Claim 19 (depends on 14) The system of claim 14, wherein the set of test data provides visual contrast between the sample and background materials. ### Claim 20 (depends on 14) The system of claim 14, wherein the storage medium contains at least one additional programming instruction that, when executed, cause the processor to perform the following: repeating the receiving, generating, and determining steps for at least one additional tuning state in response to the score image set of data not satisfying the tolerance level. ### Claim 21 (depends on 20) The system of claim 20, wherein the repeating is performed until the tolerance level is satisfied. ### Claim 22 (depends on 20) The system of claim 20, wherein the repeating is performed a pre-determined number of times or for a pre-determined amount of time. ### Claim 23 (depends on 14) The system of claim 14, wherein the first data set comprises a first image (T1) and the second data set comprises a second image (T2), and the optical computation comprises one or more of T1+T2, T1−T2, T1*T2, T1/T2, or combinations thereof. ### Claim 24 (depends on 14) The system of claim 14, wherein the tolerance level comprises at least one figure of merit. ### Claim 25 (depends on 14) The system of claim 14, wherein the at least one figure of merit comprises one or more of an area under the receiver operator characteristic (AUROC) curve, standard error of calibration (SEC), signal to noise ratio (SNR), a Fisher contrast ratio, standard error of prediction (SEP), or optical throughput (% T). ### Claim 26 (depends on 14) The system of claim 14, wherein the tunable light emitting diode has a plurality of channels corresponding to a band of light and each combination of the plurality of channels corresponds to one of the plurality of tuning states.