# US 11,306,361 B2 · Pathology prognosis method

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- Number: US 11,306,361 B2
- Document ID: US11306361B2
- Status: Granted
- Kind: B2
- Application number: 15/744,659
- Filed: 2016-07-18
- Published: 2018-07-19
- Granted: 2022-04-19
- Earliest claimed: 2015-07-16
- Applicants: Centre National De La Recherche Scientifique; Universite De Montpellier; Axlr, Satt Du Languedoc Roussillon; Institut National De La Sante Et De La Recherche Medicale (Inserm)
- Inventors: Frederic Bienvenu
- Web page: https://patentgrep.com/patent/US11306361B2

## CPC

- C12Q 1/6886: for cancer (immunoassay for cancer G01N33/575)
- A61P 35/00: Antineoplastic agents
- C12Q 1/6818: involving interaction of two or more labels, e.g. resonant energy transfer
- C12Q 1/6883: for diseases caused by alterations of genetic material
- C12Q 2600/106: Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
- C12Q 2600/118: Prognosis of disease development
- C12Q 2600/156: Polymorphic or mutational markers
- C12Q 2600/158: Expression markers
- C12Q 2600/166: Oligonucleotides used as internal standards, controls or normalisation probes
- C12Q 2600/178: miRNA, siRNA or ncRNA
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The invention relates to a method for the prognosis a person's susceptibility to environmental stress. The method includes: a step of determining the sequence for initiating the translation of at least one of the genes from the CCND family, and a step of quantifying the expression of the protein encoded by said gene, the translation initiation sequence of which was sequenced in the previous step, and comparing said expression with the expression level of said protein from a reference sample.

## Claims

### Claim 1

An in vitro method for prognosis of the resistance to chemotherapy of a tumor, said method comprising
detecting a mutation in the KOZAK sequence of the CCNDI gene by sequencing the KOZAK sequence of the CCNDI gene, said mutation being a C→G substitution in position −7 relative to the ATG codon of the sequence SEQ ID NO: 1,
wherein the sequencing is carried out using a pair of oligonucleotides as set forth in SEQ ID NO: 38 and SEQ ID NO: 39
and
concluding that the tumor will be resistant to chemotherapy.

### Claim 2

A method for the prognosis of resistance to chemotherapy of a tumor developed by an individual comprising:
identifying a mutation in the KOZAK sequence of a gene coding for a Cyclin D1 protein by sequencing the KOZAK sequence of the gene, wherein the sequencing is carried out using a pair of oligonucleotides allowing sequencing of the KOZAK sequence of the gene coding the Cyclin D1 protein wherein the pair of oligonucleotides is a pair of oligonucleotides as set forth in SEQ ID NO: 38 and SEQ ID NO: 39; and,
concluding that the tumor developed by the individual will be resistant to chemotherapy.