Methods for diagnosing infectious diseases using adsorption media
AssigneeExThera Medical Corporation
InventorsRobert S. Ward, Keith R. McCrea
Life of this patent
Estimating the term…
Abstract
The present invention provides an in vitro method for concentrating infectious pathogens found in a biological sample obtained from an individual who is suspected of being infected with the pathogens. Provided herein is also an in vitro method for reducing or eliminating blood cells from a sample obtained from an individual suspected to being infected with an infectious pathogen. The present invention also provides a method for diagnosing malaria and a method for determining if an individual is infected with a pathogen. Provided herein is also a concentrator and a kit for use with the methods.
Claims21
A method for concentrating a pathogen present in a biological sample for detection, wherein the sample is obtained from a subject who is suspected of being infected with said pathogen, the method comprising:
- (a) contacting the biological sample obtained from the subject with an adsorption media under conditions to form an adhering complex comprising the adsorption media and said pathogen, wherein the adsorption media is a solid substrate of high surface area having at least one polysaccharide adsorbent on the surface thereof, which is a member selected from the group consisting of heparin, heparan sulfate, mannose, dextran sulfate, hyaluronic acid, sialic acid, chitosan, polyethylene imine (PEI) and a combination thereof;
- (b) separating the adhering complex from components of the sample that are not included in the complex while maintaining the complex;
- (c) collecting pathogens of the adhering complex;
- and
- (d) detecting the pathogen of the adhering complex.
Dependencies are inferred from the claim text.