# US 11,306,342 B2 · Cell hydrolysate composition from cultivated cells and applications thereof

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- Number: US 11,306,342 B2
- Document ID: US11306342B2
- Status: Granted
- Kind: B2
- Application number: 17/243,493
- Filed: 2021-04-28
- Published: 2021-08-12
- Granted: 2022-04-19
- Earliest claimed: 2019-12-02
- Assignees: Avant Meats Company Limited
- Applicants: Avant Meats Company Limited
- Inventors: Po San Mario Chin; Kai Yi Carrie Chan; Chun Hei Poon
- Web page: https://patentgrep.com/patent/US11306342B2

## CPC

- C12P 21/06: produced by the hydrolysis of a peptide bond, e.g. hydrolysate products (preparing foodstuffs by protein hydrolysis A23J3/00)
- A23J 3/04: Animal proteins
- A23J 3/34: using enzymes
- A23J 3/341: {of animal proteins}
- A23J 3/342: {of collagen; of gelatin}
- A23L 33/18: Peptides; Protein hydrolysates
- A61K 8/65: Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
- A61K 8/99: from microorganisms other than algae or fungi, e.g. protozoa or bacteria
- A61K 38/18: Growth factors; Growth regulators
- A61K 38/39: Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
- A61Q 19/00: Preparations for care of the skin
- C07K 1/12: by hydrolysis {, i.e. solvolysis in general}
- C12Y 304/21001: Chymotrypsin (3.4.21.1)
- C12Y 304/21004: Trypsin (3.4.21.4)
- C12Y 304/21014: Microbial serine proteases (3.4.21.14) (C12Y304/21062 - C12Y304/21067 takes precedence)
- C12Y 304/23001: Pepsin A (3.4.23.1)
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A61K 2800/10: General cosmetic use
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- A23J: PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12Y: ENZYMES
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

A cell hydrolysate composition, the composition comprising substantially all protein polypeptides and/or polypeptide fragments derived substantially from all the proteins in a cell from an in vitro cell culture.

## Claims

### Claim 1

A cell hydrolysate composition, the composition comprising substantially (i) one or more of a mixture of protein polypeptides and/or polypeptide fragments from the group consisting of lumican, tropomyosin, chondroadherin and fibulin; and (ii) free of wastes and metabolites from a culture media used in an in vitro cell culture;
wherein the protein polypeptides and/or polypeptide fragments are obtained from non-hydrolyzed proteins released from a plurality of cells harvested from the in vitro cell culture upon lysis; and
wherein the purity of the protein polypeptides and/or polypeptide fragments in the composition is higher than that in a cell hydrolysate extracted from an animal tissue or a plant tissue.

### Claim 2 (depends on 1)

The cell hydrolysate composition of claim 1, wherein the wastes and metabolites comprise at least one of ammonia, lactate, pyruvate and putrescine.

### Claim 3 (depends on 1)

The cell hydrolysate composition of claim 1, wherein the composition is water soluble and the composition is at the pH of 6.5 to 8.5.

### Claim 4 (depends on 1)

The cell hydrolysate composition of claim 1, wherein the protein polypeptides and/or polypeptide fragments have average molecular size ranged from 100 Daltons (Da) to 500 Da.

### Claim 5 (depends on 1)

The cell hydrolysate composition of claim 1, wherein the in vitro cell culture is from an animal cell culture or a plant cell culture.

### Claim 6 (depends on 1)

The cell hydrolysate composition of claim 1, wherein the in vitro cell culture is from yellow croaker swim bladder cells.