Biosynthetic production of steviol glycoside rebaudioside D4 from rebaudioside E
AssigneeConagen Inc.
InventorsGuohong Mao, Jacob Edward Vick, Michael Batten, Yang Luo, Yilin Wu, Xiaodan Yu
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Abstract
The present invention relates, in some aspects, to the production of steviol glycoside rebaudioside D4 through the use of rebaudioside E. In some aspects, the invention relates to mutant CP1 enzymes, mutant HV1 enzymes as well as host cells and methods utilizing such enzymes, such as to produce rebaudioside D4.
Claims15
A method of producing rebaudioside D4, the method comprising incubating a substrate with a recombinant polypeptide comprising an amino acid sequence having at least 90% identity to SEQ ID NO:
- 7 or SEQ ID NO:
- 9, wherein the substrate comprises Reb E, stevioside, or combinations thereof.
A method for synthesizing rebaudioside D4 from rebaudioside E, the method comprising:
- preparing a reaction mixture comprising rebaudioside E, a substrate selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate glucose (UDP-glucose), and C1m2, and
- incubating the reaction mixture for a sufficient time to produce rebaudioside D4, wherein a glucose is covalently coupled to the rebaudioside E to produce rebaudioside D4.
A method for synthesizing rebaudioside D4 from rebaudioside E, the method comprising:
- preparing a reaction mixture comprising rebaudioside E, a substrate selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate glucose (UDP-glucose), and C1m3, and
- incubating the reaction mixture for a sufficient time to produce rebaudioside D4, wherein a glucose is covalently coupled to the rebaudioside E to produce rebaudioside D4.
Dependencies are inferred from the claim text.