# US 11,306,336 B2 · Process for production of Galacto-oligosaccharides

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- Number: US 11,306,336 B2
- Document ID: US11306336B2
- Status: Granted
- Kind: B2
- Application number: 16/477,629
- Filed: 2018-03-09
- Published: 2019-11-07
- Granted: 2022-04-19
- Earliest claimed: 2017-01-13
- Assignees: Tata Chemicals Limited
- Applicants: Tata Chemicals Limited
- Inventors: R Saravanan; S Narayanan; Deepak Jadhav; Manish Jain; Shajahan Shubethar; Shankar Lade; Manoj Gote; Ashok Kumar Dubey
- Web page: https://patentgrep.com/patent/US11306336B2

## CPC

- C12P 19/04: Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

A process for production of galacto-oligosaccharides is provided. The process for production of galacto-oligosaccharides includes growing Sporobolomyces singularis in a fermentation medium, harvesting the said microbes from the fermentation medium, inoculating the said microbes with lactose solution in a reactor and incubating in an aerobic condition to convert lactose into galacto-oligosaccharides. The process also includes separating the galacto-oligosaccharides from the microbes; filtering thus obtained galacto-oligosaccharides; and optionally making the powder form of galacto-oligosaccharides. The process for producing galacto-oligosaccharides provides high yield and high purity.

## Claims

### Claim 1

A process for producing galacto-oligosaccharides comprising:
(a) growing Sporobolomyces singularis MCC 0096 microbes in a fermentation medium;
(b) harvesting the microbes;
(c) inoculating the harvested microbes with lactose solution in a reactor in an aerobic condition, at a temperature in the range of 35 to 40° C., wherein the ratio of the said microbes with respect to lactose solution is at least 5% on w/v basis;
(d) separating the galacto-oligosaccharides from the microbes;
(e) filtering the obtained galacto-oligosaccharides of step (d);
(f) optionally making a powder form of galacto-oligosaccharides, wherein the microbes convert at least 80% of lactose into galacto-oligosaccharides.

### Claim 2 (depends on 1)

The process of claim 1, wherein the microbes are harvested from the fermentation medium at the OD620 range of 5.0-6.0.

### Claim 3 (depends on 1)

The process of claim 1, wherein microbes are grown in the fermentation medium at a temperature range of 25 to 30° C. in an aerobic condition.

### Claim 4 (depends on 1)

The process of claim 1, wherein the separation is carried out using a microfiltration system and/or centrifugation.

### Claim 5 (depends on 1)

The process of claim 1, wherein the powder is made by at least one of spray drying and crystallization.

### Claim 6 (depends on 1)

The process of claim 1, wherein the yield of galacto-oligosaccharides is at least 80%.

### Claim 7 (depends on 1)

The process of claim 1, wherein the microbes are reused.