Process of producing omega-hydroxyl fatty acid from alkane

A method of producing at least one omega-hydroxyl fatty acid, the method comprising:

• contacting at least one alkane with at least one yeast cell in an aqueous medium,
• wherein the at least one yeast cell is selected from the group consisting of Candida tropocalis with accession number ATCC20962 and Yarrowia lipolytica C122 strain with accession number CGMCC14251, and is capable of oxidizing the at least one alkane to a corresponding omega-hydroxyl fatty acid and the at least one yeast cell comprises a reduced fatty acid degradation capacity;
• the reduced fatty acid degradation capacity is a result of:
• (i) a decrease in expression relative to a wild type cell of at least one enzyme involved in a beta-oxidation pathway;
• and/or
• (ii) at least one loss-of-function mutation in at least one enzyme involved in the beta-oxidation pathway;
• and
• the aqueous medium comprises acetate as a co-substrate such that an acetate concentration of the aqueous medium is at least 20 mmol/L, and has a non-detectable concentration of glucose.