# US 11,306,310 B2 · MicroRNA inhibitor > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,306,310 B2 - Document ID: US11306310B2 - Status: Granted - Kind: B2 - Application number: 16/319,766 - Filed: 2017-07-19 - Published: 2020-03-05 - Granted: 2022-04-19 - Earliest claimed: 2016-07-19 - Assignees: Shanghai East Hospital - Applicants: Shanghai East Hospital - Inventors: Zuoren Yu; Zhongmin Liu; Qian Zhao - Web page: https://patentgrep.com/patent/US11306310B2 ## CPC - A61K 31/713: Double-stranded nucleic acids or oligonucleotides - C12N 15/113: Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} - A61P 35/00: Antineoplastic agents - C12N 2310/113: targeting other non-coding nucleic acids, e.g. antagomirs - C12N 2310/141: MicroRNAs, miRNAs - C12N 2310/3231: having an additional ring, e.g. LNA, ENA - C12N 2310/33: of the base - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS ## Abstract A microRNA (miRNA) inhibitor, being able to form a stable DNA-RNA double-stranded structure with a miRNA. The double-stranded structure comprises: (i) a miRNA unit sequence, and (ii) an RNA zipper unit sequence. The 5′-end of the RNA zipper unit sequence is connected to the 5′-end of the miRNA unit sequence. The 3′-end of the RNA zipper unit sequence is connected to the 3′-end of the miRNA unit sequence. The RNA zipper can connect the head of the miRNA molecule to the tail of the miRNA molecule to form a stable long-chain structure. The RNA zipper unit sequence contains 1-3 nucleotides not connected to the miRNA unit sequence. The DNA-RNA double-stranded structure can be used to inhibit biological activities of a miRNA unit, does not have biological toxicity, and has the advantages of high affinity, high specificity and high stability. ## Claims ### Claim 1 A small RNA inhibitor comprising a first binding region and a second binding region; the first binding region is located at 5′ end of the small RNA inhibitor, comprising an antisense complementary sequence targeting a 5′ region of a small RNA, which can bind to the 5′ region of the targeted small RNA molecule by base complementation; the second binding region is located at 3′ end of the small RNA inhibitor, comprising an antisense complementary sequence targeting a 3′ region of the small RNA, which can bind to the 3′ region of the targeted small RNA molecule by base complementation, wherein a length of the small RNA inhibitor sequence is 19-26 nt; there is a junction region between the first binding region and the second binding region, the junction region is composed of 0-3 nucleotide residues, the junction region and the first binding region and the second binding region are single-stranded; the small RNA inhibitor can bind the small RNA molecule, thereby inhibiting biological functions of the small RNA molecule, the type of small RNA molecule is selected from the group consisting of: miRNA, siRNA, and piRNA; and a length of nucleotide contained in the first binding region is N1, a length of nucleotide contained in the second binding region is N2, a length of nucleotide in the 5′ region of the small RNA molecule is N1′, a length of nucleotide in the 3′ region of the small RNA is N2′, a length of total nucleotides of the small RNA is N0, and the 5′ region of the small RNA molecule does not overlap with the 3′ region of the small RNA molecule, wherein N1′+N2′=N0; 0.3