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US 11,306,303 B2Granted
Hexuronate C4-epimerase variant having improved D-tagatose conversion activity, and D-tagatose production method using same
AssigneeCj Cheiljedang Corporation
InventorsSung Jae Yang, Young Mi Lee, Il Hyang Park, Chan-Hyoung Lee, Hyun Kug Cho, Seong Bo Kim, Yang Hee Kim, Seung Won Park
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Abstract
Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them.
Claims20
A hexuronate C4-epimerase variant comprising an amino acid sequence having at least 70% sequence identity to SEQ ID NO:
- 1, in which one or more amino acid residue selected from the group consisting of leucine (L) as an amino acid residue at position corresponding to 77, alanine (A) residue at position corresponding to 158, and proline (P) residue at position corresponding to 351, from the N-terminus of a hexuronate C4-epimerase of the amino acid sequence set forth in SEQ ID NO:
- 1 are mutated.
Dependencies are inferred from the claim text.