# US 11,306,300 B2 · Microorganism capable of displaying α-galactosidase on surface layer thereof, and use thereof

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,306,300 B2
- Document ID: US11306300B2
- Status: Granted
- Kind: B2
- Application number: 16/607,768
- Filed: 2018-05-10
- Published: 2021-09-16
- Granted: 2022-04-19
- Earliest claimed: 2017-05-11
- Assignees: Kansai Chemical Engineering Co., Ltd.; Bio-Energy Corporation
- Applicants: Kansai Chemical Engineering Co., Ltd.; Bio-energy Corporation
- Inventors: Hideo Noda; Shinji Hama
- Web page: https://patentgrep.com/patent/US11306300B2

## CPC

- C12N 9/2465: {acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)}
- C12N 9/14: Hydrolases (3)
- C12N 15/746: {for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)}
- C12N 15/81: for yeasts
- C12P 7/06: Ethanol, i.e. non-beverage
- C12P 7/56: Lactic acid
- C12P 19/02: Monosaccharides (2-ketogulonic acid C12P7/60)
- C12P 19/14: produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase}
- Y02E 50/10: Biofuels, e.g. bio-diesel
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

## Abstract

The present invention provides a transformed microorganism capable of displaying α-galactosidase on its surface layer. Also provided is a method for producing an alcohol, which includes the step of culturing the transformed microorganism in a culture medium containing a material that contains an oligosaccharide α-1,6 linked α-galactose. Also provided is a method for producing lactic acid using such a transformed microorganism together with a material that contains an oligosaccharide α-1,6 linked α-galactose. According to the present invention, a microorganism can be provided, which can degrade an oligosaccharide containing α-1,6 linked α-galactose, which may occur in soybean molasses.

## Claims

### Claim 1

A transformed microorganism capable of displaying α-galactosidase on its surface layer, wherein the α-galactosidase is α-galactosidase C (AglC) obtained from Aspergillus oryzae, and wherein a host of the microorganism is a yeast or lactic acid bacterium.

### Claim 2 (depends on 1)

The transformed microorganism according to claim 1, wherein the microorganism has an alcohol fermentation ability.

### Claim 3 (depends on 1)

The transformed microorganism according to claim 1, wherein the yeast belongs to the genus Saccharomyces, Pichia, Schizosaccharomyces, Kluyveromyces, or Candida.

### Claim 4 (depends on 3)

The transformed microorganism according to claim 3, wherein the yeast is Saccharomyces cerevisiae.

### Claim 5 (depends on 1)

The transformed microorganism according to claim 1, which is an inactivated microorganism.

### Claim 6 (depends on 1)

An α-galactosidase enzyme agent comprising the transformed microorganism according to claim 1.

### Claim 7 (depends on 2)

A method for producing alcohol, comprising:
a step of culturing the transformed microorganism according to claim 2 in a culture medium containing a material that contains an oligosaccharide containing α-1,6 linked α-galactose.

### Claim 8 (depends on 7)

The method according to claim 7, wherein the alcohol is ethanol.

### Claim 9 (depends on 1)

A method for saccharifying a material that contains an oligosaccharide containing α-1,6 linked α-galactose, the method comprising:
a step of combining the transformed microorganism according to claim 1 or the α-galactosidase enzyme agent with the material that contains the oligosaccharide containing α-1,6 linked α-galactose.

### Claim 10 (depends on 1)

A method for producing lactic acid, comprising:
a step of obtaining a saccharified material by combining the transformed microorganism according to claim 1 or the α-galactosidase enzyme agent with a material that contains an oligosaccharide containing α-1,6 linked α-galactose, and
a step of culturing a lactic acid bacterium in a culture medium containing the saccharified material.

### Claim 11 (depends on 1)

A method for producing lactic acid, comprising:
a step of culturing the transformed microorganism according to claim 1 in a culture medium containing a material that contains an oligosaccharide containing α-1,6 linked α-galactose.

### Claim 12 (depends on 7)

The method according to claim 7, wherein the oligosaccharide containing α-1,6 linked α-galactose includes at least one sugar of raffinose, stachyose, melibiose, and verbascose.