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US 11,306,293 B2Granted
Method for virus propagation
AssigneeCytiva Sweden AB
InventorsAnn-Christin Magnusson, Mats Lundgren, Eva Blanck, Christine Sund Lundstrom
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Abstract
The present invention relates to a method for virus propagation. More closely the invention relates to a method for animal component free propagation and production of rotavirus (RV) using recombinant trypsin.
Claims10
A method for rotavirus (RV) production comprising the following steps:
- cultivating cells in a bioreactor, infecting the cells with RV by activation with recombinant trypsin, and propagating the RV within said bioreactor in the presence of recombinant trypsin for maintenance/propagation, wherein the trypsin concentration is about 10 μg/ml for RV activation and about 5 μg/ml for maintenance/propagation.
A method for rotavirus (RV) production comprising the following steps:
- cultivating cells in a bioreactor, infecting the cells with RV by activation with recombinant trypsin, and propagating the RV within said bioreactor in the presence of recombinant trypsin for maintenance/propagation, wherein the cell cultivation is performed on microcarriers, and wherein the trypsin concentration is about 20 μg/ml for RV activation and about 7 μg/ml for maintenance/propagation.
Dependencies are inferred from the claim text.