Stem cells expressing mesenchymal and neuronal markers, compositions thereof, and methods of preparation thereof
AssigneesAvita International Ltd., FundaçãO Butantan
InventorsIrina Kerkis, Cristiane Valverde Wenceslau
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Abstract
The invention provides a method of producing human immature dental pulp stem cells (hIDPSCs) expressing CD44 and CD13 and lacking expression of CD146. The invention also provides compositions for use in the treatment of a neurological disease or condition selected from the group consisting of Parkinson's disease (PD), multiple sclerosis, amyotrophic lateral sclerosis (ALS), stroke, autoimmune encephalomyelitis, diabetic neuropathy, glaucomatous neuropathy, Alzheimer's disease, Huntington's disease (HD), autism, schizophrenia, stroke, ischemia, a motor disorder, and a convulsive disorder.
Claims12
A method of producing human immature dental pulp stem cells (hIDPSCs) capable of crossing the blood-brain barrier, comprising:
- a) obtaining a dental pulp (DP) from a human deciduous tooth;
- b) washing the DP with a solution containing antibiotics;
- c) establishing an explant culture by placing the DP onto a plastic surface in a culture medium;
- d) mechanically transferring the DP onto a different plastic surface in the culture medium;
- e) repeating steps c) and d);
- and
- f) collecting the hIDPSCs expressing CD44 and CD13 that adhere to the plastic surface from the explant culture, wherein at least 80% of the collected hIDPSCs express brain-derived neurotrophic factor (BDNF) and Dopamine- and cAMP-regulated phosphoprotein (DARPP-32).
Dependencies are inferred from the claim text.