# US 11,306,187 B2 · Polymer-iron oxide nano-complex, uses thereof and preparation method thereof

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- Number: US 11,306,187 B2
- Document ID: US11306187B2
- Status: Granted
- Kind: B2
- Application number: 15/901,554
- Filed: 2018-02-21
- Published: 2018-08-30
- Granted: 2022-04-19
- Earliest claimed: 2017-02-21
- Assignees: Research & Business Foundation Sungkyunkwan University
- Applicants: Research & Business Foundation Sungkyunkwan University
- Inventors: Soong-Ho Um; Seung-Won Shin; Sang-Hun Chun
- Web page: https://patentgrep.com/patent/US11306187B2

## CPC

- C08J 7/043: Improving the adhesiveness of the coatings per se, e.g. forming primers (adhesives in the form of films or foils characterised by the primer layers between the polymer carriers and the adhesives C09J7/50)
- A61K 48/0041: {the non-active part being polymeric}
- A61K 48/0083: {characterised by an aspect of the administration regime}
- A61N 2/004: {specially adapted for a specific therapy}
- C08J 3/215: at least one additive being also premixed with a liquid phase
- C08J 7/06: with compositions not containing macromolecular substances
- C09K 11/025: {non-luminescent particle coatings or suspension media}
- C09K 11/06: containing organic luminescent materials
- C12N 15/87: Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N 15/88: using microencapsulation, e.g. using {amphiphile} liposome vesicle
- C08J 2367/04: Polyesters derived from hydroxy carboxylic acids, e.g. lactones (C08J2367/06 takes precedence)
- C09K 2211/1018: Heterocyclic compounds
- C08J: WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H (working, e.g. shaping, of plastics B29)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61N: ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY (measurement of bioelectric currents A61B; surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body A61B18/00; anaesthetic apparatus in general A61M; incandescent lamps H01K; infrared radiators for heating H05B)
- C09K: MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present disclosure relates to a polymer-iron oxide composite nanoparticle, a polymer-iron oxide composite nanoparticle including a silica coating layer coated on the surface of the polymer-iron oxide composite nanoparticle, a DNA-containing polymer-iron oxide composite nanostructure including DNA attached on the silica coating layer, a method of preparing the same and a method of controlling expression of a gene.

## Claims

### Claim 1

A method of reducing expression of a gene, the method comprising:
incubating a polymer-iron oxide composite nanostructure comprising a DNA encoding the gene in an incubator comprising a DNA expression kit for the DNA at 30° C. for 8 hours, while applying a magnetic field to the polymer-iron oxide composite nanostructure by a neodymium magnet placed at a bottom of the incubator; and
confirming the reduction in gene expression when the polymer-iron oxide nanostructure is incubated in the presence of the magnetic field,
wherein the polymer-iron oxide composite nanostructure comprises;
a polymer-iron oxide composite nanoparticle;
a silica coating layer coated on a surface of the polymer-iron oxide composite nanoparticle; and
the DNA encoding the gene and attached to the silica coating layer.