# US 11,306,013 B2 · Biodegradation treatment method for organic compounds > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,306,013 B2 - Document ID: US11306013B2 - Status: Granted - Kind: B2 - Application number: 16/622,215 - Filed: 2018-06-15 - Published: 2020-06-11 - Granted: 2022-04-19 - Earliest claimed: 2017-06-19 - Assignees: Taisei Corporation - Applicants: Taisei Corporation - Inventors: Norifumi Yamamoto; Yuji Saito; Hironori Taki - Web page: https://patentgrep.com/patent/US11306013B2 ## CPC - C02F 3/34: characterised by the microorganisms used - C02F 3/348: {characterised by the way or the form in which the microorganisms are added or dosed} - B09C 1/10: microbiologically, {biologically} or by using enzymes - C12N 1/20: Bacteria; Culture media therefor - C02F 2101/34: containing oxygen - C02F 2101/366: {Dioxine; Furan} - C02F 2209/06: pH - C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes) - B09C: RECLAMATION OF CONTAMINATED SOIL (gatherers for removing stone or the like from the soil A01B43/00; sterilising soil by steam A01G11/00; removing undesirable matter, e.g. rubbish, from the land E01H15/00) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract An object is to provide an efficient treatment method for organic compounds using constitutive 1,4-dioxane-degrading bacterium strain N23. As a means for achieving the object, a biodegradation treatment method is provided, wherein organic compounds are biodegradation-treated with strain N23, which is a constitutive 1,4-dioxane-degrading bacterium deposited under Accession No. NITE BP-02032, under a condition of pH 3.0 or higher but no higher than 5.5. ## Claims ### Claim 1 A biodegradation treatment method comprising biodegradation-treating at least one organic compound, to reduce concentration thereof, with strain N23, which is a constitutive 1,4-dioxane-degrading bacterium deposited under Accession No. NITE BP-02032, under a condition of pH 3.8 or higher but no higher than 5.5, which is lower than an optimal pH for 1,4-dioxane degradation activity and growth of the bacterium, while maintaining 80% or greater of degradation activity at pH 7.0 and maintaining the capability of growth, wherein the organic compound is selected from the group consisting of cyclic ethers, ethylene glycol, diethylene glycol, 1,4-butanediol, and combinations thereof. ### Claim 2 (depends on 1) The biodegradation treatment method according to claim 1, wherein the organic compound is selected from the group consisting of 1,4-dioxane, 1,3-dioxolane, 2-methyl-1,3-dioxolane, tetrahydrofuran, and combinations thereof. ### Claim 3 (depends on 1) The biodegradation treatment method according to claim 1, wherein a carbon source selected from the group consisting of ethylene glycol, diethylene glycol, 1,4-butanediol, 1,4-dioxane, and combinations thereof is added to the organic compound. ### Claim 4 (depends on 1) The biodegradation treatment method according to claim 1, which is performed by a fed-batch process comprising: (i) adding strain N23 to a liquid containing the organic compound, (ii) conducting biodegradation of the organic compound for a given time period, (iii) after step (ii), replacing a part of a supernatant which does not contain strain N23 with a fresh liquid containing the organic compound, and (iv) repeating steps (ii) and (iii) for a given time period. ### Claim 5 (depends on 1) The biodegradation treatment method according to claim 1, which is performed by a continuous process. ### Claim 6 (depends on 2) The biodegradation treatment method according to claim 2, wherein a carbon source selected from the group consisting of ethylene glycol, diethylene glycol, 1,4-butanediol, 1,4-dioxane, and combinations thereof is added to the organic compound. ### Claim 7 (depends on 2) The biodegradation treatment method according to claim 2, which is performed by a fed-batch process comprising: (i) adding strain N23 to a liquid containing the organic compound, (ii) conducting biodegradation of the organic compound for a given time period, (iii) after step (ii), replacing a part of a supernatant which does not contain strain N23 with a fresh liquid containing the organic compound, and (iv) repeating steps (ii) and (iii) for a given time period. ### Claim 8 (depends on 2) The biodegradation treatment method according to claim 2, which is performed by a continuous process. ### Claim 9 (depends on 3) The biodegradation treatment method according to claim 3, which is performed by a fed-batch process comprising: (i) adding strain N23 to a liquid containing the organic compound, (ii) conducting biodegradation of the organic compound for a given time period, (iii) after step (ii), replacing a part of a supernatant which does not contain strain N23 with a fresh liquid containing the organic compound, and (iv) repeating steps (ii) and (iii) for a given time period. ### Claim 10 (depends on 3) The biodegradation treatment method according to claim 3, which is performed by a continuous process.