# US 11,304,991 B2 · Antimicrobial compositions and methods of use thereof

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- Number: US 11,304,991 B2
- Document ID: US11304991B2
- Status: Granted
- Kind: B2
- Application number: 16/947,808
- Filed: 2020-08-18
- Published: 2021-01-07
- Granted: 2022-04-19
- Earliest claimed: 2011-10-07
- Assignees: Morehouse School of Medicine
- Applicants: Morehouse School of Medicine
- Inventors: Vincent C. Bond; Michael Powell; Ming Bo Huang; Syed Ali; Martin N. Shelton
- Web page: https://patentgrep.com/patent/US11304991B2

## CPC

- A61K 38/162: {from virus}
- A61K 38/08: Peptides having 5 to 11 amino acids {(A61K38/043 - A61K38/046 take precedence)}
- A61K 45/06: Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
- A61K 47/60: the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
- A61P 31/00: Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P 31/04: Antibacterial agents
- C07K 7/06: having 5 to 11 amino acids
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- C12N 2740/16032: Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present application discloses a method for treating microbial infection using an antimicrobial composition comprises antimicrobial peptide which contains at least one VGFPV motif.

## Claims

### Claim 1

A method of treating a gram positive or gram negative bacterial infection, comprising:
administering to a subject in need thereof an effective amount of an antimicrobial peptide comprising the amino acid sequence of SEQ ID NO: 3 and a cell penetrating peptide (CPP) domain.

### Claim 2 (depends on 1)

The method of claim 1, wherein said antimicrobial peptide comprises the amino acid sequence of SEQ ID NO: 5.

### Claim 3 (depends on 1)

The method of claim 1, wherein said antimicrobial peptide further comprises a second antimicrobial domain, wherein said second antimicrobial domain is different from said first antimicrobial domain.

### Claim 4 (depends on 1)

The method of claim 1, wherein said antimicrobial peptide is administered in combination with another antimicrobial agent.

### Claim 5 (depends on 4)

The method of claim 4, wherein said another antimicrobial agent is another antimicrobial peptide.

### Claim 6 (depends on 4)

The method of claim 4, wherein said antimicrobial peptide is a PEGylated peptide.

### Claim 7

A method of treating a gram positive or gram negative bacterial infection, comprising:
administering to a subject in need of such treatment an effective amount of an in vivo expression vector that encodes an antimicrobial peptide comprising the amino sequence of SEQ ID NO: 3 and a cell penetrating peptide (CPP) domain,
wherein said in vivo expression vector expresses said antimicrobial peptide in said subject.

### Claim 8 (depends on 7)

The method of claim 7, wherein said antimicrobial peptide comprises the amino acid sequence of SEQ ID NO: 5.