# US 11,304,388 B2 · Method for promoting growth and bioactive substances of Crepidiastrum denticulatum > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,304,388 B2 - Document ID: US11304388B2 - Status: Granted - Kind: B2 - Application number: 16/097,139 - Filed: 2017-04-26 - Published: 2019-05-23 - Granted: 2022-04-19 - Earliest claimed: 2016-04-28 - Assignees: Seoul Viosys Co., Ltd.; Industry-University Cooperation Foundation of Chungbuk National University - Applicants: Seoul Viosys Co., Ltd.; Industry-University Cooperation Foundation of Chungbuk National University - Inventors: Myung Min Oh; Song Yi Park; Jin Hui Lee; Ji Hoon Bae; Jong Hyun Koo - Web page: https://patentgrep.com/patent/US11304388B2 ## CPC - A01G 7/00: Botany in general - A01G 22/60: Flowers; Ornamental plants - A01G 3/00: Cutting implements specially adapted for horticultural purposes; Delimbing standing trees (forestry delimbers A01G23/095) - A01G 7/04: Electric or magnetic {or acoustic} treatment of plants for promoting growth - A01G 7/045: {with electric lighting} - A01H 6/14: Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce - Y02P 60/14: Measures for saving energy, e.g. in green houses - A01G: HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING (picking of fruits, vegetables, hops or the like A01D46/00; propagating unicellular algae C12N1/12) - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES - Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS ## Abstract A method for promoting growth and bioactive substances of Crepidiastrum denticulatum including performing a stress treatment on Crepidiastrum denticulatum during cultivation thereof, in which the stress treatment includes at least of applying visible light, drying, exposing to low temperature, irradiating ultraviolet rays, and applying a chemical elicitor. ## Claims ### Claim 1 A method for promoting growth and bioactive substances of Crepidiastrum denticulatum, the method comprising: performing a stress treatment on Crepidiastrum denticulatum during cultivation thereof, the stress treatment comprising at least one of applying visible light, drying, exposing to low temperature, irradiating ultraviolet rays, and applying a chemical elicitor. ### Claim 2 (depends on 1) The method of claim 1, wherein the stress treatment comprises applying visible light, the visible light is at least one of white light, red light, green light, and blue light. ### Claim 3 (depends on 2) The method of claim 2, wherein the visible light is one monochromatic light of red light, green light, and blue light. ### Claim 4 (depends on 2) The method of claim 2, wherein the visible light comprises mixed light having the ratio of at least one of: red light:blue light=6:4; red light:blue light=7:3; red light:blue light=8:2; red light:blue light=9:1; red light:green light:blue light=5:1:4; red light:green light:blue light=6:1:3; red light:green light:blue light=7:1:2; red light:green light:blue light=8:1:1; red light:green light:blue light=9:1:0; red light:green light:blue light=7:2:1; red light:white light:blue light=8:1:1; red light:white light:blue light=6:2:2; red light:white light:blue light=7:1:2; and red light:white light:blue light=8:2:0. ### Claim 5 (depends on 4) The method of claim 4, wherein the mixed light further comprises a far-red light. ### Claim 6 (depends on 5) The method of claim 5, wherein the mixed light comprises a red light and a blue light mixed at a ratio of 8:2. ### Claim 7 (depends on 6) The method of claim 6, wherein a ratio of the red light to the far-red light is at least one of 1.7, 1.2, 4.1, and 8.6 to 1. ### Claim 8 (depends on 5) The method of claim 5, further comprising harvesting Crepidiastrum denticulatum on the 6th week after planting Crepidiastrum denticulatum, when the mixed light comprising the far red light is applied during cultivation. ### Claim 9 (depends on 1) The method of claim 1, further comprising harvesting Crepidiastrum denticulatum on the 6th week after planting Crepidiastrum denticulatum, when the visible light is applied during cultivation. ### Claim 10 (depends on 1) The method of claim 1, wherein the stress treatment comprises drying, the drying comprises stop watering Crepidiastrum denticulatum for a predetermined period of time. ### Claim 11 (depends on 10) The method of claim 10, further comprising harvesting Crepidiastrum denticulatum on the 3rd to 5th days after the drying stress treatment. ### Claim 12 (depends on 1) The method of claim 1, wherein the stress treatment comprises drying, the drying comprises supplying water to Crepidiastrum denticulatum for a predetermined period of time using a wick. ### Claim 13 (depends on 12) The method of claim 12, further comprising harvesting Crepidiastrum denticulatum on the 2nd day after the drying stress treatment. ### Claim 14 (depends on 1) The method of claim 1, wherein the stress treatment comprises exposing to low temperature, the low temperature stress treatment comprises exposing Crepidiastrum denticulatum at 10° C. during one of night time and day time. ### Claim 15 (depends on 1) The method of claim 1, wherein the stress treatment comprises exposing to low temperature, further comprising harvesting Crepidiastrum denticulatum on the 3rd to 5th days after the low temperature stress treatment. ### Claim 16 (depends on 1) The method of claim 1, wherein the stress treatment comprises irradiating ultraviolet rays, the ultraviolet rays comprises UV. ### Claim 17 (depends on 16) The method of claim 16, further comprising harvesting Crepidiastrum denticulatum 8 hours after UV-A is irradiated to Crepidiastrum denticulatum. ### Claim 18 (depends on 1) The method of claim 1, wherein the stress treatment comprises irradiating ultraviolet rays, the ultraviolet rays comprises UV-B. ### Claim 19 (depends on 18) The method of claim 18, further comprising harvesting Crepidiastrum denticulatum on the 2nd day after UV-B is irradiated to Crepidiastrum denticulatum. ### Claim 20 (depends on 19) The method of claim 19, wherein UV-B is irradiated to Crepidiastrum denticulatum for 1 hour in every 11 hours during 2 days. ### Claim 21 (depends on 1) The method of claim 1, wherein the stress treatment comprises applying the chemical elicitor, the chemical elicitor comprises salicylic acid. ### Claim 22 (depends on 21) The method of claim 21, further comprising harvesting Crepidiastrum denticulatum on the 3rd day after the chemical elicitor is sprayed onto Crepidiastrum denticulatum. ### Claim 23 (depends on 1) The method of claim 1, wherein Crepidiastrum denticulatum after the stress treatment has an increase in at least one of a shoot fresh weight, a shoot dry weight, number of leaves, a leaf area, a leaf length, and a leaf width of an aerial part. ### Claim 24 (depends on 1) The method of claim 1, wherein the bioactive substances of Crepidiastrum denticulatum promoted by the stress treatment comprise a phenolic compound and a chicoric acid.