Protein reporters for ultrasensitive detection methods
AssigneeThe Trustees of the University of Pennsylvania
InventorsIvan J. Dmochowski, Yanfei Wang, Benjamin W. Roose, Serge Zemerov, Zhuangyu Zhao
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Abstract
The present invention relates to compositions and methods using protein reporters as imaging agents in 129Xe NMR and MRI applications. It is described that bla and MBP are genetically-encoded proteins that induce a detectable chemical shift during 129Xe NMR, allowing for use as protein reporters in research and clinical applications.
Claims11
An 129Xe NMR imaging agent comprising one or more protein reporters selected from the group consisting of TEM-1 β-lactamase (bla), and a mutant of bla, wherein the mutant of bla comprises a point mutation located at a residue selected from the group consisting of 1263, 1279, 1282, M182 and a combination thereof.
A method of 129Xe NMR imaging comprising the steps of:
- a. administering to a cell or subject a 129Xe NMR imaging agent comprising one or more protein reporters selected from the group consisting of bla and a mutant of bla, wherein the mutant of bla comprises a point mutation located at a residue selected from the group consisting of I263, I279, I282, M182 and a combination thereof;
- b. administering hyperpolarized xenon to the cell or subject;
- c. obtaining magnetic resonance data of a target site of the cell or subject;
- and
- d. analyzing the data to produce a magnetic resonance image of the target site.
Dependencies are inferred from the claim text.