# US 11,299,747 B2 · Transgenic plants having altered biomass composition

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- Number: US 11,299,747 B2
- Document ID: US11299747B2
- Status: Granted
- Kind: B2
- Application number: 16/992,793
- Filed: 2020-08-13
- Published: 2021-03-18
- Granted: 2022-04-12
- Earliest claimed: 2011-12-09
- Assignees: Ceres, Inc.
- Applicants: Ceres, Inc.
- Inventors: Joon-Huyn Park; Roger I. Pennell; Richard Flavell; Chuan-Yin Wu
- Web page: https://patentgrep.com/patent/US11299747B2

## CPC

- C12N 15/8297: {Gibberellins; GA3}
- C07K 14/415: from plants
- C12N 15/8245: {involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis}
- C12N 15/8246: {Non-starch polysaccharides, e.g. cellulose, fructans, levans}
- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Methods and materials for modulating biomass composition in plants are disclosed. For example, nucleic acids encoding biomass composition-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having altered biomass composition and plant products produced from plants having altered biomass composition.

## Claims

### Claim 1

A rice plant comprising an exogenous nucleic acid said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide having 90 percent or greater sequence identity to the amino acid sequence of SEQ ID NO:473, wherein the rice plant is selected for having a biomass with increased saccharification efficiency relative to the corresponding saccharification efficiency of a biomass of a control plant that does not comprise said exogenous nucleic acid.

### Claim 2 (depends on 1)

The rice plant of claim 1, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:473.

### Claim 3

A method of producing a rice plant, said method comprising
growing rice plant comprising an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide having 90 percent or greater sequence identity to the amino acid sequence of SEQ ID NO:473,
selecting the rice plant for having increased saccharification efficiency as compared to the corresponding saccharification efficiency of a control plant that does not comprise said nucleic acid.

### Claim 4 (depends on 1)

The rice plant of claim 1, wherein the plant is selected for having a biomass with increased saccharification efficiency, resulting in an at least 2.0 fold increase in glucose, fructose, and sucrose, relative to the saccharification efficiency of a biomass of a control plant that does not comprise said nucleic acid, and wherein the regulatory region is heterologous with respect to the nucleotide sequence.

### Claim 5 (depends on 4)

The rice plant of claim 4, wherein the plant is selected for having an increase in sugar concentration relative to a control plant that does not comprise said nucleic acid.

### Claim 6 (depends on 4)

The rice plant of claim 4, wherein the plant is further selected for having an increase in plant height relative to a control plant that does not comprise said nucleic acid.

### Claim 7 (depends on 3)

The method of claim 3, wherein selecting said plant comprises selecting said plant for having an increase in sugar concentration relative to a control plant that does not comprise said nucleic acid.

### Claim 8 (depends on 7)

The method of claim 7, wherein the plant is selected for having an at least a 1.5 fold, a 2.0 fold, or a 2.5 fold increase in glucose from cell wall as compared to that of a control plant that does not comprise said exogenous nucleic acid.

### Claim 9 (depends on 7)

The method of claim 7, wherein the plant is selected for having an at least a 3 fold, a 4 fold, or a 6 fold increase in sugar yield as compared to that of a control plant that does not comprise said exogenous nucleic acid.

### Claim 10 (depends on 1)

A progeny of the rice plant of claim 1, wherein the progeny comprises the exogenous nucleic acid.

### Claim 11 (depends on 1)

A seed producing the rice plant of claim 1.