# US 11,299,746 B2 · Disease resistant pepper plants > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,299,746 B2 - Document ID: US11299746B2 - Status: Granted - Kind: B2 - Application number: 16/921,770 - Filed: 2020-07-06 - Published: 2020-10-22 - Granted: 2022-04-12 - Earliest claimed: 2014-06-18 - Assignees: Enza Zaden Beheer B.V. - Applicants: Enza Zaden Beheer B.V. - Inventors: Christianus Cornelis Nicolaas Van Schie; Karin Ingeborg Posthuma; Tieme Zeilmaker; Geert Johannes De Boer - Web page: https://patentgrep.com/patent/US11299746B2 ## CPC - C12N 15/8282: {for fungal resistance} - A01H 5/08: Fruits - A01H 6/822: {Capsicum sp. [pepper]} - C07K 14/415: from plants - C12N 9/0071: {acting on paired donors with incorporation of molecular oxygen (1.14)} - C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance} - C12Q 1/6895: for plants, fungi or algae - C12Q 2600/13: Plant traits - C12Q 2600/156: Polymorphic or mutational markers - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES ## Abstract The present invention relates to Phytophthora capsici resistant Capsicum annuum plants wherein said resistance is conferred by a combination of two genes. The present invention further relates to the use of these genes for providing Phytophthora capsici resistant Capsicum annuum plants and proteins encoded by the present genes. ## Claims ### Claim 1 An isolated Capsicum annuum pepper plant resistant to Phytophthora capsici, wherein the pepper plant comprises a mutated first coding sequence comprising SEQ ID NO: 29 and comprises a mutated second coding sequence comprising SEQ ID NO: 32, and wherein the plant exhibits resistance to Phytophthora capsici. ### Claim 2 (depends on 1) The pepper plant of claim 1, further comprising a mutated first protein comprising SEQ ID NO: 25 and a mutated second protein comprising SEQ ID NO: 28. ### Claim 3 (depends on 1) A seed, tissue, or plant part of the pepper plant of claim 1, wherein the seed, tissue, or plant part comprises the mutated first coding sequence and the mutated second coding sequence. ### Claim 4 (depends on 3) The seed, tissue, or plant part of claim 3, wherein the seed, tissue, or plant part further comprises a mutated first protein comprising SEQ ID NO: 25, and a mutated second protein comprising SEQ ID NO: 28. ### Claim 5 (depends on 1) A method for obtaining the isolated Capsicum annuum pepper plant of claim 1, comprising introducing a mutation into a coding sequence of a first gene to produce a mutated first coding sequence comprising SEQ ID NO: 29 and introducing a mutation into a coding sequence of a second gene to produce a mutated second coding sequence comprising SEQ ID NO: 32, wherein the mutated first coding sequence encodes a mutated first protein and the mutated second coding sequence encodes a mutated second protein. ### Claim 6 (depends on 5) The method of claim 5, wherein the mutated first protein comprises SEQ ID NO: 25 and the mutated second protein comprises SEQ ID NO: 28. ### Claim 7 An isolated Capsicum annuum pepper plant resistant to Phytophthora capsici, wherein the pepper plant comprises a mutated first protein comprising SEQ ID NO: 25 and a mutated second protein comprising SEQ ID NO: 28, and wherein the plant exhibits resistance to Phytophthora capsici. ### Claim 8 (depends on 7) The pepper plant of claim 7, further comprising a mutated first coding sequence comprising SEQ ID NO: 29 and a mutated second coding sequence comprising SEQ ID NO: 32. ### Claim 9 (depends on 7) A seed, tissue, or plant part of the pepper plant of claim 7, wherein the seed, tissue, or plant part comprises the mutated first protein and the mutated second protein. ### Claim 10 (depends on 9) The seed, tissue, or plant part of claim 9, wherein the seed, tissue, or plant part further comprises a mutated first coding sequence comprising SEQ ID NO: 29 and a mutated second coding sequence comprising SEQ ID NO: 32.