# US 11,299,728 B2 · Sample extraction and preparation device > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,299,728 B2 - Document ID: US11299728B2 - Status: Granted - Kind: B2 - Application number: 16/898,865 - Filed: 2020-06-11 - Published: 2020-11-26 - Granted: 2022-04-12 - Earliest claimed: 2013-11-01 - Assignees: Biomeme, Inc. - Applicants: Biomeme, Inc. - Inventors: Marc Dominic DeJohn; Jesse Wilson vanWestrienen - Web page: https://patentgrep.com/patent/US11299728B2 ## CPC - C12N 15/1017: {by filtration, e.g. using filters, frits, membranes} - B01L 3/0217: {of the plunger pump type} - B01L 3/502: {with fluid transport, e.g. in multi-compartment structures} - B01L 2200/0631: Purification arrangements, e.g. solid phase extraction [SPE] - B01L 2300/0681: Filter - B01L 2400/0478: pistons - B01L 2400/049: vacuum - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ## Abstract A fluid sample extraction device is disclosed comprising a housing defining an internal fluid passage having a distal open end and a proximal open end and a porous medium within the fluid passage, between the distal open end and proximal open end. The porous medium, which may be glass, is configured to allow fluid flow in the fluid passage to flow around the porous medium, toward the proximal open end of the housing, when fluid is drawn from the distal open end toward the proximal open end, and to allow fluid in the fluid passage to flow toward the distal open end of the housing, through the porous medium when the fluid is forced toward the distal open end. The porous medium is also configured to capture nucleic acids in the porous medium from the fluid when fluid is forced through the porous medium. Devices and kits are also disclosed. ## Claims ### Claim 1 A fluid extraction device, comprising: a housing having a fluid passage and a porous medium freely movable in said fluid passage, wherein said porous medium is capable of moving, relative to said housing, between a first position to open a space adjacent to said porous medium and a second position to close said space adjacent to said porous medium; and a pressure source in fluid communication with said fluid passage, wherein said pressure source is configured to: (i) direct fluid into said fluid passage through said space adjacent to said porous medium, wherein upon said fluid being directed into said fluid passage, said porous medium is positioned in said first position; and (ii) discharge said fluid from said fluid passage through said porous medium, wherein upon said fluid being discharged from said fluid passage, (1) said porous medium is positioned in said second position, thereby closing said space adjacent to said porous medium, and (2) said porous medium captures nucleic acids from said fluid. ### Claim 2 (depends on 1) The fluid extraction device of claim 1, wherein said porous medium comprises at least one material selected from the group consisting of silica, borosilicate, polyethylene, nylon, and Acrylonitrile-Butadiene-Styrene (ABS). ### Claim 3 (depends on 1) The fluid extraction device of claim 1, wherein said fluid extraction device further comprises a proximal fitting, a distal fitting, and a chamber therebetween, wherein said chamber is in fluid communication with said fluid passage, wherein said chamber comprises a proximal surface and a distal surface, and wherein said chamber comprises said porous medium. ### Claim 4 (depends on 3) The fluid extraction device of claim 3, wherein said chamber further comprises a lateral surface, wherein at least a portion of said lateral surface comprises a taper relative to a longitudinal axis of said fluid passage. ### Claim 5 (depends on 4) The fluid extraction device of claim 4, wherein a size of said space adjacent to said porous medium is based at least in part on an angle of said taper. ### Claim 6 (depends on 1) The fluid extraction device of claim 1, wherein said fluid extraction device further comprises a tip with an opening in fluid communication with said fluid passage. ### Claim 7 (depends on 3) The fluid extraction device of claim 3, wherein at least one of said proximal surface and said distal surface comprises one or more grooves that is configured to distribute said fluid in said chamber upon flow of said fluid towards said chamber. ### Claim 8 (depends on 1) The fluid extraction device of claim 1, wherein said porous medium comprises two or more portions. ### Claim 9 (depends on 8) The fluid extraction device of claim 8, wherein said two or more portions of said porous medium comprises a first portion and a second portion, wherein said first portion comprises glass, and wherein said second portion comprises a polymer. ### Claim 10 (depends on 1) The fluid extraction device of claim 1, wherein said porous medium is freely movable relative to said housing in its entirety. ### Claim 11 (depends on 1) The fluid extraction device of claim 1, wherein said porous medium comprises glass. ### Claim 12 (depends on 1) The fluid extraction device of claim 1, wherein said porous medium comprises a polymer. ### Claim 13 (depends on 1) The fluid extraction device of claim 1, wherein said porous medium has a pore size from about 0.2 micrometers to 3 micrometers. ### Claim 14 (depends on 1) The fluid extraction device of claim 1, wherein said pressure source is a syringe. ### Claim 15 (depends on 1) The fluid extraction device of claim 1, further comprising a disk adjacent to said porous medium. ### Claim 16 (depends on 15) The fluid extraction device of claim 15, wherein said disk is porous. ### Claim 17 (depends on 15) The fluid extraction device of claim 15, wherein said disk is non-porous. ### Claim 18 (depends on 15) The fluid extraction device of claim 15, wherein said disk is configured to prevent a material of said porous medium from obstructing of said fluid passage. ### Claim 19 (depends on 1) The fluid extraction device of claim 1, wherein said porous medium is capable of moving between said first position and said second position upon deformation of at least a portion of said porous medium. ### Claim 20 (depends on 1) The fluid extraction device of claim 1, wherein said porous medium is capable of moving between said first position and said second position by translation.