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US 11,299,723 B2Granted
Engineered beta-glucosidases and glucosylation methods
AssigneeCodexis, Inc.
InventorsJack Liang, Jonathan Vroom, Stephanie Sue Galanie, Nikki Dellas, Ravi David Garcia
Life of this patent
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Abstract
The present invention provides engineered β-glucosidase (BGL) enzymes, polypeptides having BGL activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the enzymes, and methods of using the engineered BGL enzymes to make products with β-glucose linkages.
Claims32
A non-naturally occurring beta-glucosidase variant having at least 80% sequence identity to SEQ ID NO:
- 14, wherein said variant comprises at least one substitution or substitution set at one or more positions 15, 16, 16/84, 17, 19, 21, 35, 45, 55, 76, 79, 121, 164, 168, 168/256, 170, 179, 215/413, 221, 221/311, 225, 247, 313, 351, 356, 402, 404, 405, 409, 411, 412, 413, and 414, wherein said positions are numbered with reference to SEQ ID NO:14.
Dependencies are inferred from the claim text.