Production of fully functional mature beta cells from human pancreatic progenitors
AssigneeThe Regents of the University of California
InventorsMatthias Hebrok, Holger Andreas Russ, Gopika G. Nair
Life of this patent
Estimating the term…
Abstract
Methods are provided for the simple, fast, effective and safe directed differentiation of embryonic stem cells into the mature beta cells of enriched beta clusters, wherein the beta cells rapidly and reliably secrete insulin in response to glucose levels. The cells are useful transplant therapeutics for diabetic individuals. These cells can also be used for drug screening purposes to identify factors/chemicals capable of increasing beta cell functions, proliferation, survival, and resistance to immune assault.
Claims14
A method of producing human endocrine progenitor cells from pluripotent stem cells comprising:
- (a) incubating pluripotent stem cells (PSCs) in culture medium comprising Wnt3a, Activin A, TGβi, and keratinocyte growth factor (KGF) for 3-7 days;
- (b) exposing the cells resulting from step (a) to medium comprising retinoic acid (RA) for 2-3 days;
- and
- (c) culturing the cells resulting from step (b) in medium comprising RA, epidermal growth factor (EGF), and KGF,
- thereby producing a culture comprising greater than 70% PDX1+ NKX6.1+ human endocrine progenitor cells.
Dependencies are inferred from the claim text.