# US 11,299,521 B2 · Polypeptide and method of producing IMP using the same

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- Number: US 11,299,521 B2
- Document ID: US11299521B2
- Status: Granted
- Kind: B2
- Application number: 16/346,041
- Filed: 2018-12-14
- Published: 2020-12-03
- Granted: 2022-04-12
- Earliest claimed: 2017-12-15
- Assignees: Cj Cheiljedang Corporation
- Applicants: Cj Cheiljedang Corporation
- Inventors: Jung Gun Kwon; Min Ji Baek; Ji Hye Lee; Nara Kwon; Ju Jeong Kim; Jin Ah Rho; Jin Man Cho
- Web page: https://patentgrep.com/patent/US11299521B2

## CPC

- C07K 14/34: from Corynebacterium (G)
- C12N 1/20: Bacteria; Culture media therefor
- C12N 1/205: Bacterial isolates
- C12N 15/77: for Corynebacterium; for Brevibacterium
- C12P 19/32: having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
- C12R 2001/15: Corynebacterium
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present disclosure relates to a novel polypeptide having an activity of exporting 5′-inosine monophosphate, a microorganism comprising the same, a method for preparing 5′-inosine monophosphate using the same, and a method for increasing export of 5′-inosine monophosphate.

## Claims

### Claim 1

A protein variant exporting 5′-inosine monophosphate, comprising a polypeptide having at least 95% sequence identity to SEQ ID NO: 1, wherein the 164th amino acid in the amino acid sequence of SEQ ID NO: 1 is substituted with another amino acid at the corresponding position in the protein variant, wherein the protein variant has 5′-inosine monophosphate exporting activity.

### Claim 2 (depends on 1)

The protein variant of claim 1, wherein the another amino acid is selected from the group consisting of lysine, arginine, asparagine, glycine, threonine, and proline.

### Claim 3 (depends on 1)

A polynucleotide encoding the protein variant of claim 1.

### Claim 4 (depends on 3)

A vector comprising the polynucleotide of claim 3.

### Claim 5 (depends on 1)

A microorganism of genus Corynebacterium producing 5′-inosine monophosphate, which comprises the protein variant of claim 1; a polynucleotide encoding the protein variant of claim 1; or a vector comprising the polynucleotide encoding the protein variant of claim 1.

### Claim 6 (depends on 5)

The microorganism of the genus Corynebacterium of claim 5, wherein the microorganism of the genus Corynebacterium is Corynebacterium stationis.

### Claim 7 (depends on 5)

A method for preparing 5′-inosine monophosphate, comprising culturing the microorganism of the genus Corynebacterium of claim 5 in a medium; and recovering 5′-inosine monophosphate from the microorganism or the medium.

### Claim 8 (depends on 7)

The method of claim 7, wherein the microorganism of the genus Corynebacterium is Corynebacterium stationis.