# US 11,299,439 B2 · Mycorrhizae and/or Bacillus amyloliquefaciens liquid fertilizer compatible formulations

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,299,439 B2
- Document ID: US11299439B2
- Status: Granted
- Kind: B2
- Application number: 16/837,058
- Filed: 2020-04-01
- Published: 2020-10-01
- Granted: 2022-04-12
- Earliest claimed: 2019-04-01
- Assignees: Valent BioSciences Corporation
- Applicants: Valent BioSciences LLC
- Inventors: Bala N. Devisetty
- Web page: https://patentgrep.com/patent/US11299439B2

## CPC

- C05F 11/08: Organic fertilisers containing added bacterial cultures, mycelia or the like
- A01G 7/06: Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
- A01N 63/22: Bacillus
- A01N 63/30: Microbial fungi; Substances produced thereby or obtained therefrom
- A01P 21/00: Plant growth regulators
- C05G 3/50: Surfactants; Emulsifiers
- C05G 5/20: Liquid fertilisers (layered or coated C05G5/30; incorporated into a matrix C05G5/40)
- C05G 5/23: Solutions
- C12N 1/04: Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00)
- C12R 2001/07: Bacillus
- C12R 2001/645: Fungi {; Processes using fungi}
- C05F: ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- A01G: HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING (picking of fruits, vegetables, hops or the like A01D46/00; propagating unicellular algae C12N1/12)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01P: BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- C05G: MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS (organic fertilisers containing added bacterial cultures, mycelia, or the like C05F11/08; organic fertilisers containing plant vitamins or hormones C05F11/10); FERTILISERS CHARACTERISED BY THEIR FORM
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present invention is directed to agricultural formulations containing mycorrhizae and/or Bacillus amyloliquefaciens that are compatible with liquid fertilizers. The present invention further relates to methods of improving plant growth by applying mycorrhizae and/or Bacillus amyloliquefaciens containing formulations to an area where a plant will grow.

## Claims

### Claim 1

An agricultural formulation comprising:
an active ingredient selected from the group consisting of mycorrhizae, Bacillus amyloliquefaciens and a mixture thereof;
propylene glycol; and
a stabilizer selected from the group consisting of benzenesulfonic acid, decyl (sulfophenoxy)-disodium salt, benzenesulfonic acid oxybis(decyl)-disodium salt mixture, sodium mono- and di-decyl disulfonated diphenyl oxide, D-glucopyranose, oligomeric, C9-11-alkyl glycosides and a mixture thereof.

### Claim 2 (depends on 1)

The formulation of claim 1, further comprising alkyl quaternary ammonium clay.

### Claim 3 (depends on 1)

The formulation of claim 1, wherein the Bacillus amyloliquefaciens is strain BA-1.

### Claim 4 (depends on 1)

The formulation of claim 1, further comprising a liquid fertilizer.

### Claim 5 (depends on 4)

The formulation of claim 4, wherein the liquid fertilizer is a 10-34-0 liquid fertilizer.

### Claim 6 (depends on 4)

The formulation of claim 4, wherein the formulation comprises from about 80% to about 99% v/v of liquid fertilizer, wherein v/v denotes volume by total volume of the formulation.

### Claim 7

An agricultural formulation comprising:
an ingredient selected from the group consisting of about 0.1% to about 1% w/w mycorrhizae, from about 1% to about 5% w/w Bacillus amyloliquefaciens and a mixture thereof;
from about 70% to about 97% w/w propylene glycol; and
from about 1% to about 20% w/w of a stabilizer selected from the group consisting of benzenesulfonic acid, decyl (sulfophenoxy)-disodium salt, benzenesulfonic acid oxybis(decyl)-disodium salt mixture, sodium mono- and di-decyl disulfonated diphenyl oxide, D-glucopyranose, oligomeric, C9-11-alkyl glycosides and a mixture thereof, wherein w/w denotes weight by total weight of the formulation.

### Claim 8 (depends on 7)

The formulation of claim 7, further comprising from about 1% to about 5% w/w of alkyl quaternary ammonium clay.

### Claim 9 (depends on 8)

The formulation of claim 8, comprising:
about 0.25% w/w mycorrhizae;
about 92.5% w/w propylene glycol;
about 5.0% w/w of a stabilizer selected from the group consisting of benzenesulfonic acid, decyl (sulfophenoxy)-disodium salt, benzenesulfonic acid oxybis(decyl)-disodium salt mixture, sodium mono- and di-decyl disulfonated diphenyl oxide, D-glucopyranose, oligomeric, C9-11-alkyl glycosides and a mixture thereof; and
about 2.2% w/w alkyl quaternary ammonium clay.

### Claim 10 (depends on 8)

The formulation of claim 8, comprising:
about 3.0% w/w Bacillus amyloliquefaciens;
about 88.75% w/w propylene glycol;
about 5.0% w/w of sodium mono- and di-decyl disulfonated diphenyl oxide; and
about 3.25% w/w alkyl quaternary ammonium clay.

### Claim 11 (depends on 8)

The formulation of claim 8, comprising:
about 0.27% w/w mycorrhizae;
about 3.0% w/w Bacillus amyloliquefaciens;
about 88.48% w/w propylene glycol;
about 5.0% w/w of sodium mono- and di-decyl disulfonated diphenyl oxide; and
about 3.25% w/w alkyl quaternary ammonium clay.

### Claim 12 (depends on 7)

A tank mix comprising from about 1% to about 20% v/v of the formulation of claim 7 and from about 80% to about 99% v/v of a liquid fertilizer, wherein v/v denotes volume by total volume of the tank mix.

### Claim 13 (depends on 12)

The tank mix of claim 12, wherein the liquid fertilizer is a 10-34-0 liquid fertilizer.

### Claim 14 (depends on 1)

A method of improving plant growth comprising applying a formulation of claim 1 to an area where a plant will grow.

### Claim 15 (depends on 14)

The method of claim 14, wherein the area where a plant will grow is soil.

### Claim 16 (depends on 15)

The method of claim 15, wherein the soil is in the form of a furrow.

### Claim 17 (depends on 14)

The method of claim 14, wherein the area where a plant will grow is a plant root zone.