# US 11,298,700 B2 · Feedback system for parallel droplet control in a digital microfluidic device > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,298,700 B2 - Document ID: US11298700B2 - Status: Granted - Kind: B2 - Application number: 16/726,740 - Filed: 2019-12-24 - Published: 2020-08-13 - Granted: 2022-04-12 - Earliest claimed: 2016-08-22 - Assignees: mirOculus Inc. - Applicants: mirOculus Inc. - Inventors: Ik Pyo Hong; Irena Barbulovic-Nad; Jorge Abraham Soto-Moreno - Web page: https://patentgrep.com/patent/US11298700B2 ## CPC - B01L 3/502792: {for moving individual droplets on a plate, e.g. by locally altering surface tension} - B01L 2300/165: Specific details about hydrophobic, oleophobic surfaces - B01L 2300/1822: using Peltier elements - B01L 2300/1894: Cooling means; Cryo cooling - B01L 2400/0424: Dielectrophoretic forces - B01L 2400/0427: Electrowetting - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ## Abstract Digital microfluidics apparatuses (e.g., devices and systems) configured to determine provide feedback on the location, rate of movement, rate of evaporation and/or size (or other physical characteristic) of one or more, and preferably more than one, droplet in the gap region of a digital microfluidics (DMF) apparatus. ## Claims ### Claim 1 A digital microfluidic (DMF) apparatus, the apparatus comprising: a plurality of actuation electrodes configured to move one or more droplets when actuated; a ground electrode; a voltage source coupled to the ground electrode; a plurality of sensing circuits, each of the plurality of sensing circuits comprising a charging circuit and a discharging circuit, wherein each sensing circuit is electrically connected to a corresponding actuation electrode of the plurality of actuation electrodes, and wherein each sensing circuit is configured to detect a charged voltage of a capacitor in the charging circuit of the sensing circuit; and a controller configured to alternately provide voltage from the voltage source to the ground electrode and one or more actuation electrodes of the plurality of actuation electrodes to move the one or more droplets, further wherein the controller is configured to sense, in parallel, one or more properties of the one or more droplets based on input from the plurality of sensing circuits when applying voltage to the ground electrode. ### Claim 2 (depends on 1) The apparatus of claim 1, wherein the sensed one or more properties include at least one of: a location of the one or more droplets relative to the plurality of actuation electrodes, a rate of movement of the one or more droplets, a rate of evaporation of the one or more droplets, or a size of the one or more droplets. ### Claim 3 (depends on 1) The apparatus of claim 1, wherein the discharging circuit comprises a transistor and a ground. ### Claim 4 (depends on 1) The apparatus of claim 1, wherein the charging circuit comprises a capacitor and a diode. ### Claim 5 (depends on 1) The apparatus of claim 1, further comprising an analog-to-digital converter (ADC) wherein the ADC is configured to detect the charged voltage of the charging circuit. ### Claim 6 (depends on 5) The apparatus of claim 5, wherein the controller is configured to sequentially activate the discharging circuit, then the charging circuit, and to receive the charged voltage of the charging circuit from the ADC in parallel for all of the sensing circuits of the plurality of sensing circuits. ### Claim 7 (depends on 1) The apparatus of claim 1, further comprising a forward/reverse switch connected between the voltage source, the ground electrode, and the plurality of actuation electrodes, wherein the controller is configured to operate the forward/reverse switch to switch between providing voltage to one or more of the plurality of electrodes and the ground electrode. ### Claim 8 (depends on 1) The apparatus of claim 1, further comprising a plurality of electrode switches, wherein each electrode switch of the plurality of electrode switches is connected to an actuation electrode of the plurality of actuation electrodes and is controlled by the controller through a switch controller to apply voltage from the voltage source to the actuation electrode. ### Claim 9 (depends on 1) The apparatus of claim 1, wherein the controller is configured to compare a voltage sensed by each of the plurality of sensing circuits to a threshold voltage value to determine the property of the one or more droplets. ### Claim 10 (depends on 1) The apparatus of claim 1, wherein the controller is configured to compare a voltage sensed by each of the plurality of sensing circuits to a predetermined voltage value or range of voltage values to determine the property of the one or more droplets wherein the property comprises a size of one or more droplets. ### Claim 11 A method of simultaneously determining one or more properties of multiple drops in a digital microfluidics (DMF) apparatus, the method comprising: applying voltage to a plurality of actuation electrodes to move one or more droplets within a gap between the plurality of actuation electrodes and one or more ground electrodes; applying voltage to one or more of the one or more ground electrodes; concurrently sensing, in a plurality of sensing circuits, wherein each sensing circuit of the plurality of sensing circuits is associated with an actuation electrode of the plurality of actuation electrodes, a charging voltage while applying voltage to the one or more ground electrodes; and determining the one or more properties of the one or more droplets based on the sensed charging voltages by comparing the sensed charging voltages to a predetermined value or range of values, wherein the one or more properties includes one or more of: a location of the one or more droplets relative to the plurality of actuation electrodes, a rate of movement of the one or more droplets, a rate of evaporation of the one or more droplets, or a size of the one or more droplets. ### Claim 12 (depends on 11) The method of claim 11, wherein applying voltage to the plurality of actuation electrodes and applying voltage to the one or more ground electrodes comprises applying voltage from the same high voltage source. ### Claim 13 (depends on 11) The method of claim 11, wherein applying voltage to the plurality of actuation electrodes comprises sequentially applying voltage to adjacent actuation electrodes. ### Claim 14 (depends on 11) The method of claim 11, further comprising re-applying voltage to one or more of the plurality of actuation electrodes based on a determined location of the one or more droplets. ### Claim 15 (depends on 11) The method of claim 11, wherein applying voltage to one or more ground electrodes comprises applying voltage to the one or more ground electrodes without applying voltage to the plurality of actuation electrodes. ### Claim 16 (depends on 11) The method of claim 11, further comprising discharging voltage in each of the sensing circuits prior to applying voltage to the one or more ground electrodes. ### Claim 17 (depends on 11) The method of claim 11, further comprising charging a capacitor in each of the sensing circuits of the plurality of sensing circuits when applying voltage to the one or more ground electrodes. ### Claim 18 (depends on 11) The method of claim 11, further comprising discharging voltage in each of the sensing circuits prior to applying voltage to the one or more ground electrodes and then charging a capacitor in each of the sensing circuits in the plurality of sensing circuits when applying voltage to the one or more ground electrodes.