# US 11,298,698 B2 · Method and system for performing heat assisted biochemical reactions

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- Number: US 11,298,698 B2
- Document ID: US11298698B2
- Status: Granted
- Kind: B2
- Application number: 16/353,907
- Filed: 2019-03-14
- Published: 2019-09-19
- Granted: 2022-04-12
- Earliest claimed: 2018-03-15
- Assignees: Kryptos Biotechnologies, Inc.
- Applicants: Kryptos Biotechnologies, Inc.
- Inventors: Jun Ho Son; Sewoon Han; Jinyong Lee
- Web page: https://patentgrep.com/patent/US11298698B2

## CPC

- B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
- B01L 3/567: {Valves, taps or stop-cocks}
- B01L 7/52: {with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples}
- B01L 7/525: {with physical movement of samples between temperature zones}
- B01L 7/54: {using spatial temperature gradients}
- B01L 9/527: {for microfluidic devices, e.g. used for lab-on-a-chip}
- B01L 2200/0689: Sealing
- B01L 2200/10: Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
- B01L 2300/044: pierceable, e.g. films, membranes
- B01L 2300/0645: Electrodes
- B01L 2300/0663: Whole sensors
- B01L 2300/0681: Filter
- B01L 2300/0829: Multi-well plates; Microtitration plates
- B01L 2300/0864: comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
- B01L 2300/123: Flexible; Elastomeric
- B01L 2300/168: Specific optical properties, e.g. reflective coatings
- B01L 2300/18: Means for temperature control
- B01L 2300/1805: Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L 2300/185: using a liquid as fluid
- B01L 2300/1861: using radiation
- B01L 2400/06: Valves, specific forms thereof
- B01L 2400/0666: Solenoid valves
- C12Q 1/6844: Nucleic acid amplification reactions
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Systems and methods for light based heating of light absorbing sources for modification of nucleic acids through fast thermal cycling of polymerase chain reaction are described.

## Claims

### Claim 1

A system comprising:
a transparent block comprising one or more concave intrusions;
a light absorbing film deposited within the one or more concave intrusions of the transparent block;
a reaction vessel removably positioned onto the concave intrusions of the transparent block;
a sealing film disposed on the reaction vessel; and
a light source;
wherein the light source is configured to be directed at the concave intrusions of the transparent block such that light from the light source passes through the transparent block to the light absorbing film in the concave intrusions to generate heat within the light absorbing film and subsequent heating of the reaction vessel.

### Claim 2 (depends on 1)

The system of claim 1 wherein the transparent block comprises a fluid circulation channel.

### Claim 3 (depends on 2)

The system of claim 2 wherein at least one of air, water, or a liquid flows through the fluid circulation channel.

### Claim 4 (depends on 1)

The system of claim 1 wherein the sealing film comprises a light absorbing layer.

### Claim 5 (depends on 1)

The system of claim 1 further comprising a high refractive index material disposed outside of the transparent block for internal reflection of light from an excitation LED.

### Claim 6 (depends on 1)

The system of claim 1 further comprising a first filter for emission of a fluorescent dye and a second filter for elimination of light from the light source.

### Claim 7 (depends on 1)

The system of claim 1 further comprising a sealing plate with a light absorbing material on a surface of the sealing plate.

### Claim 8 (depends on 1)

The system of claim 1 wherein the light source is a pulsed light source.

### Claim 9 (depends on 8)

The system of claim 8 further comprising a detector operable to detect a signal indicating nucleic acids modification during an off cycle of the pulsed light source.

### Claim 10

A system comprising:
a polymeric reaction vessel comprising one or more wells;
a light absorbing film deposited within the one or more wells of the polymeric reaction vessel;
a transparent block with concave intrusions to hold the polymeric reaction vessel;
a light source configured to be directed at the concave intrusions of the transparent block such that light from the light source passes through the transparent block to the light absorbing film in the concave intrusions to generate heat within the light absorbing film and heats the polymeric reaction vessel; and
a sealing film disposed on the polymeric reaction vessel.

### Claim 11 (depends on 10)

The system of claim 10 wherein an additional channel is placed around the concave intrusions.

### Claim 12 (depends on 10)

The system of claim 10 wherein one or more concave intrusions in the wells comprise 2-D or 3-D microstructures or nanostructures in the form of a pillar array, 1D or 2D grating, photonic crystal, or hemi-sphere.

### Claim 13 (depends on 10)

The system of claim 10 wherein the transparent block comprises a fluid circulation channel.

### Claim 14 (depends on 13)

The system of claim 13 wherein at least one of air, water, or a liquid flows through the circulation channel.

### Claim 15 (depends on 10)

The system of claim 10 wherein an emission wavelength of the light source does not overlap with an excitation wavelength of a fluorescent dye used for real-time detection of nucleic acids.

### Claim 16 (depends on 10)

The system of claim 10 wherein the sealing film comprises a light absorbing layer.

### Claim 17 (depends on 10)

The system of claim 10 further comprising a high refractive index material disposed outside of the transparent block, wherein the high refractive index material is operable to reflect light from an excitation LED.

### Claim 18 (depends on 10)

The system of claim 10 further comprising a first filter for emission of fluorescent dye and a second filter for elimination of light from the light source.

### Claim 19 (depends on 10)

The system of claim 10 further comprising a sealing plate with a light absorbing material on a surface of the sealing plate.

### Claim 20 (depends on 10)

The system of claim 10 wherein the light source comprises a pulsed light source.