# US 11,298,696 B2 · Reaction cassette and assay device > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,298,696 B2 - Document ID: US11298696B2 - Status: Granted - Kind: B2 - Application number: 16/668,396 - Filed: 2019-10-30 - Published: 2020-05-14 - Granted: 2022-04-12 - Earliest claimed: 2018-11-08 - Assignees: Apex Biotechnology Corp. - Applicants: Apex Biotechnology Corp. - Inventors: Sz Hau Chen; Ming Chang Hsu; Yu-Ju Lu; Chia-Jung Wang; Jui-Pin Hrian - Web page: https://patentgrep.com/patent/US11298696B2 ## CPC - B01L 3/5023: {with a sample being transported to, and subsequently stored in an absorbent for analysis} - B01L 3/502707: {characterised by the manufacture of the container or its components} - B01L 3/50273: {characterised by the means or forces applied to move the fluids} - B01L 3/502746: {characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves} - B01L 3/502753: {characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation} - B01L 3/502761: {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules} - B01L 2200/0621: Control of the sequence of chambers filled or emptied - B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions - B01L 2300/0858: Side walls - B01L 2300/087: Multiple sequential chambers - B01L 2300/0874: Three dimensional network - B01L 2300/089: Virtual walls for guiding liquids - B01L 2400/0457: passive flow or gravitation - B01L 2400/086: using baffles or other fixed flow obstructions - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ## Abstract The present disclosure provides a reaction cassette for biochemical test. The reaction cassette includes a structural wall, a first flow guiding member and an obstacle member. The structural wall defines a reaction region and a channel region, wherein the reaction region is connected to the channel region. The first flow guiding member is disposed in the channel region, and a first angle between the structural wall and the first flow guiding member ranges between 0 and 60 degrees. The obstacle member is disposed on the structural wall, and a second angle between the obstacle member and the structural wall is greater than 90 degrees. The present disclosure further provides an assay device including said reaction assay for biochemical test. ## Claims ### Claim 1 A reaction cassette for biochemical test, comprising: a structural wall, defining a reaction region and a channel region, the reaction region being connected to the channel region; a first flow guiding member, disposed in and extending along the channel region, wherein an angle between the structural wall and the first flow guiding member ranges between 0 and 80 degrees; and an obstacle member, disposed on the structural wall, wherein an angle between the obstacle member and the structural wall is greater than 90 degrees, wherein the obstacle member protrudes toward the first flow guiding member and the channel region. ### Claim 2 (depends on 1) The reaction cassette according to claim 1, wherein the first flow guiding member and the obstacle member are spaced by a vertical distance. ### Claim 3 (depends on 2) The reaction cassette according to claim 2, wherein the vertical distance ranges between 0.1 and 4 mm. ### Claim 4 (depends on 1) The reaction cassette according to claim 1, wherein the first flow guiding member and the structural wall are separated. ### Claim 5 (depends on 1) The reaction cassette according to claim 1, wherein a length of the first flow guiding member is greater than 4 mm and less than a length of the channel region. ### Claim 6 (depends on 1) The reaction cassette according to claim 1, further comprising: a second flow guiding member, disposed in the channel region, wherein an angle between the structural wall and the second flow guiding member ranges between 0 and 80 degrees. ### Claim 7 (depends on 6) The reaction cassette according to claim 6, wherein the second flow guiding member and the first flow guiding member are separated, and an extension direction of the second flow guiding member is different from an extension direction of the first flow guiding member. ### Claim 8 (depends on 6) The reaction cassette according to claim 6, wherein the second flow guiding member and the first flow guiding member are separated and parallel to each other. ### Claim 9 An assay device for biochemical test, comprising: a reaction cassette, comprising: a housing; a structural wall, disposed in the housing, defining a reaction region and a channel region, the reaction region being connected to the channel region, the channel region extending along a first direction; a flow guiding member, disposed in the channel region and extending along the first direction; and an obstacle member, disposed on the structural wall and protruding toward the first guiding member and the channel region; a storage member, configured to assemble with the reaction cassette so as to enable a specimen to flow into the reaction cassette to undergo a reaction; and a sampling member, configured to assemble with the reaction cassette to absorb a reacted specimen. ### Claim 10 (depends on 9) The assay device according to claim 9, wherein the housing is square in shape, and the first direction is parallel to a side of the housing. ### Claim 11 (depends on 9) The assay device according to claim 9, wherein the flow guiding member is a protruding structure protruding from the housing toward the channel region. ### Claim 12 (depends on 9) The assay device according to claim 9, wherein the flow guiding member is a groove structure recessed from a surface of the housing facing the channel region. ### Claim 13 (depends on 9) The assay device according to claim 9, wherein the housing comprises a front cover and a back cover, and the flow guiding member comprises a first part on the front cover and a second part on the back cover. ### Claim 14 (depends on 13) The assay device according to claim 13, wherein the first part and the second part are opposite to each other, and a gap is formed between the first part and the second part for the specimen to flow through. ### Claim 15 (depends on 13) The assay device according to claim 13, wherein the structural wall is parallel to one side of the housing, and the obstacle member is disposed on the back cover and is configured to define an assembly slot corresponding to an assembly bolt of the front cover.