# US 11,298,563 B2 · Bactericidal methods and compositions > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,298,563 B2 - Document ID: US11298563B2 - Status: Granted - Kind: B2 - Application number: 17/387,579 - Filed: 2021-07-28 - Published: 2021-11-18 - Granted: 2022-04-12 - Earliest claimed: 2019-01-31 - Assignees: Pulsethera, Inc. - Applicants: Pulsethera, Inc. - Inventors: Pu-Ting Dong; Jie Hui; Ji-Xin Cheng; Yifan Zhu - Web page: https://patentgrep.com/patent/US11298563B2 ## CPC - A61K 45/06: Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca - A61N 5/062: {Photodynamic therapy, i.e. excitation of an agent} - A01N 25/00: Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application {, e.g. seed treatment or sequential application}; Substances for reducing the noxious effect of the active ingredients to organisms other than pests - A01N 59/00: Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds - A61K 31/7036: having at least one amino group directly attached to the carbocyclic ring, e.g. streptomycin, gentamycin, amikacin, validamycin, fortimicins - A61K 31/7048: having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin {, digitoxin or digoxin} - A61K 33/38: Silver; Compounds thereof - A61K 33/40: Peroxides - A61L 2/10: Ultraviolet [UV] radiation - A61L 2/208: Hydrogen peroxide - A61N 5/0616: {Skin treatment other than tanning} - A61N 5/0624: {for eliminating microbes, germs, bacteria on or in the body} - A61N 5/067: using laser light - A61L 2101/02: Inorganic materials - A61L 2103/50: Textiles, e.g. bedwear or towels - A61N 2005/0635: {characterised by the body area to be irradiated} - A61N 2005/0643: {Applicators, probes irradiating specific body areas in close proximity} - A61N 2005/0651: {Diodes} - A61N 2005/0662: {Visible light} - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61N: ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY (measurement of bioelectric currents A61B; surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body A61B18/00; anaesthetic apparatus in general A61M; incandescent lamps H01K; infrared radiators for heating H05B) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) ## Abstract Methods of the present invention comprise photoinactivation of catalase in combination with low-concentration peroxide solutions and/or ROS generating agents to provide antibacterial effects. ## Claims ### Claim 1 A method of treating a tissue of a subject infected with a catalase-positive microbe, said method comprising the steps of: applying visible light to the tissue of the subject infected with the catalase-positive microbe at a wavelength of about 400 nm to about 430 nm, wherein the catalase is inactivated, and subsequently contacting the tissue with a composition comprising a diluted peroxide solution, thereby treating the tissue of the subject infected with the catalase-positive microbe. ### Claim 2 (depends on 1) The method of claim 1, wherein the wavelength is about 410 nm. ### Claim 3 (depends on 1) The method of claim 1, wherein the dose of the light is about 5 J/cm2 to about 200 J/cm2. ### Claim 4 (depends on 1) The method of claim 1, wherein the dose of the light is about 15 J/cm2. ### Claim 5 (depends on 1) The method of claim 1, wherein the catalase-positive microbe is a fungal or bacterial microbe and the light is provided by a pulsed nanosecond laser. ### Claim 6 (depends on 1) The method of claim 1, wherein the catalase-positive microbe is a fungal or bacterial microbe and the light is provided by a continuous wave LED. ### Claim 7 (depends on 1) The method of claim 1, wherein the diluted peroxide solution is a hydrogen peroxide solution. ### Claim 8 (depends on 7) The method of claim 7, wherein the hydrogen peroxide solution is between about 0.03% and about 0.3% hydrogen peroxide. ### Claim 9 (depends on 8) The method of claim 8, wherein the hydrogen peroxide solution is 0.3% hydrogen peroxide. ### Claim 10 (depends on 1) The method of claim 1, further comprising administering a reactive oxygen species generating agent to the infected tissue of the subject. ### Claim 11 (depends on 1) The method of claim 1, wherein the tissue is skin, scalp or nails. ### Claim 12 A method of producing a synergistic antimicrobial effect in a tissue of a subject infected with a catalase-positive microbe, said method comprising the steps of: applying visible light to the tissue of the subject infected with the catalase-positive microbe at a wavelength of about 400 nm to about 460 nm, wherein the catalase is inactivated, and subsquently contacting the tissue with a composition comprising a diluted peroxide solution, thereby producing the synergistic antimicrobial effect in the tissue of the subject infected with the catalase-positive microbe. ### Claim 13 A method of treating a tissue of a subject infected with a catalase-positive microbe, said method comprising the steps of: applying visible light to the tissue of the subject infected with the catalase-positive microbe at a wavelength of about 400 nm to about 430 nm, wherein the catalase is inactivated, and subsequently contacting the tissue with a composition comprising a reactive oxygen species generating agent, thereby treating the tissue of the subject infected with the catalase-positive microbe. ### Claim 14 (depends on 13) The method of claim 13, wherein the wavelength is about 410 nm. ### Claim 15 (depends on 13) The method of claim 13, wherein the dose of the light is about 5 J/cm2 to about 200 J/cm2. ### Claim 16 (depends on 13) The method of claim 13, wherein the dose of the light is about 15 J/cm2. ### Claim 17 (depends on 13) The method of claim 13, wherein the catalase-positive microbe is a fungal or bacterial microbe and the light is provided by a pulsed nanosecond laser. ### Claim 18 (depends on 13) The method of claim 13, wherein the catalase-positive microbe is a fungal or bacterial microbe and the light is provided by a continuous wave LED. ### Claim 19 (depends on 13) The method of claim 13, further comprising administering a diluted peroxide solution to the infected tissue of the subject. ### Claim 20 (depends on 13) The method of claim 13, wherein the tissue is skin, scalp or nails.