# US 11,298,416 B2 · Modified Brucella vaccine strain for the treatment of brucellosis

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,298,416 B2
- Document ID: US11298416B2
- Status: Granted
- Kind: B2
- Application number: 16/766,629
- Filed: 2018-11-26
- Published: 2021-03-18
- Granted: 2022-04-12
- Earliest claimed: 2017-11-24
- Assignees: Consejo Superior De Investigaciones Cientificas (Csic); Universidad Publica de Navarra
- Applicants: Consejo Superior De Investigaciones CientíFicas (Csic); Universidad PúBlica De Navarra
- Inventors: María Jesús Grilló Dolset; Beatriz San Román Aberasturi; Leyre Palacios Chaves; Sara Mena Bueno; Ana Zabalza Baranguá
- Web page: https://patentgrep.com/patent/US11298416B2

## CPC

- A61K 39/098: {Brucella}
- A61P 31/04: Antibacterial agents
- A61K 2039/522: {avirulent or attenuated}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present application provides a modified Brucella strain, its use as a medicament, and its use as a medicament for the treatment and/or prevention of brucellosis. The Brucella strain has been modified through an inactivation of the wzm gene. Further, the present application provides a pharmaceutical composition which comprises the modified Brucella strain, its use as a medicament, and its use as a medicament for the treatment and/or prevention of brucellosis. The present application also provides a kit which comprises the modified Brucella strain and a pharmaceutically acceptable carrier or diluent and its use for the treatment and/or prevention of brucellosis.

## Claims

### Claim 1

A method for prevention of brucellosis, comprising administering to a subject a modified Brucella melitensis Rev1 strain, wherein the wzm gene has been inactivated, wherein the method is for prevention of brucellosis in cattle, goats, sheep, pigs, dogs, or combinations thereof, and the brucellosis is caused by an infectious agent selected from the group consisting of B. melitensis, B. abortus and B. ovis.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the wzm gene has been partially deleted.

### Claim 3 (depends on 2)

The method according to claim 2, wherein at least 50% of SEQ ID NO: 1 has been deleted.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the strain has been further modified to express a fluorescent protein.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the strain has been lyophilized.